We narrowed to 7,609 results for: Pol
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Plasmid#196871PurposeExpression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from GolgiDepositorInsertAcGFP-Akap9 (128-425) (Akap9 Rat)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Nedd1-mOrange2-hCM1
Plasmid#196863PurposeExpression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRCDepositorInsertNedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motor
Plasmid#191330PurposeExpresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
ExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644Available SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N116C-G193C
Plasmid#162580PurposeExpresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationAsparagine 116 was mutated to Cysteine and Glycin…PromoterpolyhedrinAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-Kif13B tail 442-1843
Plasmid#162721PurposeMammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residuesDepositorInsertKif 13B (Kif13b Mouse)
ExpressionMammalianMutationWT with deletion of the 441 N-terminal residues.PromoterChicken Beta actinAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP5
Plasmid#128939PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, ZDepositorInsertsTagsHisExpressionBacterialMutationResidues 234-241 are substituted for a PreScissio…PromoterT7Available SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A-R69A
Plasmid#127787PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32, Arg36 and Arg69 to AlaAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-BC0491-luc2
Plasmid#227133PurposeBarcoded assay, cAMP/Ca2+ sensor; barcode BC0491DepositorInsert6x clustered CRE element linked to CMV minimal promoter driving barcode 0491 and a luciferase reporter gene
ExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ER-RE_MLPmin_BC1065-luc2
Plasmid#227111PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1065DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1014-luc2
Plasmid#227108PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1014DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ER-RE_MLPmin_BC1063-luc2
Plasmid#227109PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1063DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMVmin-BC0538-luc2
Plasmid#227140PurposeBarcoded assay, Ca2+ sensor; barcode BC05038DepositorInsert6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-AP1x3-GM55
Plasmid#217414PurposeLuciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseExpressionMammalianPromoter3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…Available SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-dCas13b-ADAR2DD
Plasmid#190913PurposeAddgene #103871 with 3x FLAG on N-terminal of dCas13DepositorInsert3X FLAG
UseCRISPRTags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-29Q
Plasmid#84350PurposeExpression of the human Huntingtin Exon1 protein containing 29Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-CD69 CaRE3 (minus strand)
Plasmid#91845PurposeLuciferase reporter for CD69 enhancer (IGI-P0622)DepositorInsertCD69 CaRE3 (minus strand) (CD69 Human)
UseLuciferaseExpressionMammalianMutationMissing 1 base from 13 base polyT tract beginning…Available SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
TagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable SinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat
Plasmid#42568DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTMiniTrex-mCherry-Shark2
Plasmid#233144PurposeServes as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassetteDepositorInsertsmCherry
Shark2
UseProkaryotic expressionTags3x Ty tagMutationCodon optimized for Trypanosoma cruziAvailable SinceMarch 20, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits