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We narrowed to 7,654 results for: Pol

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Showing: 4141 - 4160 of 7654 results

  4. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

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  5. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162685-plasmid-map-sequence-id-320886

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  7. pGBW-m4046219

    Plasmid
    #153926
    Purpose
    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153926-plasmid-map-sequence-id-300491

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  8. pGBW-m4046319

    Plasmid
    #153866
    Purpose
    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153866-plasmid-map-sequence-id-300389

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  9. DEP-GFP

    Plasmid
    #109043
    Purpose
    Expression of the Dishevelled DEP domain
    Depositor
    Mariann Bienz
    Publication
    Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dishevelled segment polarity protein 2 (DVL2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Dvl2 DEP domain
    Promoter
    CMV
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    109043-plasmid-map-sequence-id-220610

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  11. RIPK2 (RIPK2A-c036)

    Plasmid
    #110268
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RIPK2 (RIPK2 Human)
    Tags
    His-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110268-plasmid-map-sequence-id-212280

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  12. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

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  13. pMpGWB222

    Plasmid
    #68613
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Publication
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68613-plasmid-map-sequence-id-121832

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  15. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

    Plasmid
    #46839
    Purpose
    Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated
    Depositor
    Phil Sharp
    Publication
    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    46839-plasmid-map-sequence-id-66684

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  19. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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Showing: 4141 - 4160 of 7654 results