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YCe2730 HC_Kan_conn_BsaI-4_p25

Plasmid

#100671

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1759 HC_Kan_CAGp_p3

Plasmid

#100672

Purpose

part designed to occupy position 3 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

CAG promoter

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CTL2

Plasmid

#92212

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJL006

Plasmid

#92329

Purpose

Express mEos3.2 tagged FtsZD212A mutant in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Mutation

D212A

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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B33

Plasmid

#74008

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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B51

Plasmid

#74005

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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B53

Plasmid

#74006

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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B31

Plasmid

#74007

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TryX

Plasmid

#89623

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Tryparedoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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Stagia3-Cre

Plasmid

#73991

Purpose

Cre in Stagia3 backbone; vector-Cre

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Cre

Tags

Myc

Expression

Mammalian

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pstb-LAFR5-exoY

Plasmid

#86170

Purpose

pstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/Bam

Depositor

Kay Jones

Article

Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

Insert

exoY

Expression

Bacterial

Promoter

Smb21651

Available Since

Feb. 6, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pBP-TL6

Plasmid

#72990

Purpose

RBS_M3

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL7

Plasmid

#72991

Purpose

RBS_M5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL9

Plasmid

#72993

Purpose

RBS_L2

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L2 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-TL10

Plasmid

#72994

Purpose

RBS_L3

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL12

Plasmid

#72996

Purpose

RBS_L5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-BBa_B0012

Plasmid

#72997

Purpose

BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_B0012 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-L3S2P21

Plasmid

#72999

Purpose

L3S2P21 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3S2P21 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-L3S1P51

Plasmid

#73000

Purpose

L3S1P51 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3S1P51 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-L3S1P32

Plasmid

#73001

Purpose

L3S1P32 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3S1P32 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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