We narrowed to 39,104 results for: Eras

1. LRTi

   Plasmid

   #214072

   Purpose

   Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTcI42-Wasabi

   Plasmid

   #86118

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI42
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, K6N, S33T, Y61H, L119P, F122C

   Promoter

   pR/pL and pR/pL, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPO GFP

   Plasmid

   #68488

   Purpose

   TOPO construct expressing GFP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Green Fluorescent Protein
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. LRTX_ctrl

   Plasmid

   #214073

   Purpose

   Luminescent reporter system, NMD negative control. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Remnant of linker sequence present at the end of …

   Promoter

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTetO-ISce1-TetO

   Plasmid

   #58506

   Purpose

   ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   Tet repeats flanking ISce1 restriction site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PUMA Frag1-Luc

   Plasmid

   #16591

   Depositor

   Bert Vogelstein

   Article

   Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

   Insert

   PUMA promotor fragment (BBC3 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-PLE-IRES-mCherry

   Plasmid

   #45766

   Depositor

   Luke Lavis

   Article

   Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

   Insert

   esterase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK1024 (pDS-Def1)

   Plasmid

   #183125

   Purpose

   Expresses double-stranded RNA targeting the Def1 gene from Apis mellifera

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

   Insert

   Fragment of Def1 gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFPL-Rh

   Plasmid

   #61649

   Purpose

   Gateway vector for generating red fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   monomeric red fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VPS35 shRNA2

    Plasmid

    #163859

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV0679

    Plasmid

    #149689

    Purpose

    Bacterial expression of SENP_EuH protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuH protease
    

    Use

    Tags

    His14-tag and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LRT_RTctrl

    Plasmid

    #214074

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LRT

    Plasmid

    #214071

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 6xO/S luc

    Plasmid

    #69445

    Purpose

    detection/assessment of OCT4 and SOX2 transcription factor activity

    Depositor

    Lisa Dailey

    Insert

    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ateam1.03-nD/nA/pRSETB

    Plasmid

    #51960

    Purpose

    bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ateam1.03-nD/nA/pENTR1A

    Plasmid

    #51959

    Purpose

    Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. SARS-CoV-2 Spike-d18

    Plasmid

    #164583

    Purpose

    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

    Depositor

    Jason Sheltzer

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1256-1273

    Promoter

    EF1a

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTcI-Wasabi

    Plasmid

    #86101

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL2B -938/+64

    Plasmid

    #24943

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

21. JD638

    Plasmid

    #160395

    Purpose

    Vitis miR396-resistant GRF4-GIF1 in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    Vitis miR396-resistant GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDUP3

    Plasmid

    #92174

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PP244

    Plasmid

    #78978

    Purpose

    Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTM1_L_ZEBOV

    Plasmid

    #69120

    Purpose

    Encodes for Ebola Virus Zaire L under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    L ZEBOV (L )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCBFRE-(mt)-luc

    Plasmid

    #26896

    Depositor

    Nicholas Gaiano

    Article

    Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

    Insert

    CBFRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated CBF1 sites (see note)

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pDN-LexAOx4-mNeonGreen

    Plasmid

    #192604

    Purpose

    Vector for inducible mNeonGreen expression with 4x LexA operator (LexAO)

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    mNeonGreen
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Minimal promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRF TpC hTERT intron-1

    Plasmid

    #171070

    Purpose

    Firefly-Renilla luciferase assay for hTERT promoter activity of hTERT intron-1

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    hTERT intron-1
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-Sfp R4-4 mutant

    Plasmid

    #173491

    Purpose

    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

    Depositor

    Jun Yin

    Article

    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

    Insert

    Sfp-R44
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMB1610_pRR-Puro

    Plasmid

    #65853

    Purpose

    Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

    Depositor

    Marc Bühler

    Article

    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

    Insert

    split puromycin N-acetyltransferase coding sequence
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-EF1a-V5-LIC

    Plasmid

    #120247

    Purpose

    For LIC cloning with N-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge