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We narrowed to 63,680 results for: cloning

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Showing: 4161 - 4180 of 63680 results

  3. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid
    #111731
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q145 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111731-plasmid-map-sequence-id-226895

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  7. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  8. pKK-FLAG-BirA-TEV

    Plasmid
    #105770
    Purpose
    Expression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    FLAG-BirA-TEV
    Expression
    Mammalian
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    105770-plasmid-map-sequence-id-203337

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  9. xCas9(3.6)-BE4

    Plasmid
    #108386
    Purpose
    Mammalian expression vector for xCas9(3.6)-BE4
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-BE4
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108386-plasmid-map-sequence-id-207182

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  12. pCAGGS_3E5E_eGFP

    Plasmid
    #103054
    Purpose
    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Publication
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    Tags
    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Dec. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    103054-plasmid-map-sequence-id-197128

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  16. pCAG Cas9-2A-Citrine

    Plasmid
    #92358
    Purpose
    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 2A Citrine
    Expression
    Mammalian
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92358-plasmid-map-sequence-id-178229

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  19. pY31

    Plasmid
    #84746
    Purpose
    Expresses huLbCpf1-P2A-puro and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    huLbCpf1
    puromycin resistance gene
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84746-plasmid-map-sequence-id-162433

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Showing: 4161 - 4180 of 63680 results