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Plasmid#110159PurposeLentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cellsDepositorInsertTFAP4 (transcription factor AP-4) (TFAP4 Human)
UseLentiviralTagsHAExpressionMammalianMutationE135A/S139APromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta with CaMKKalpha RP domain
Plasmid#33332DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationCaMKKbeta RP domain (203-225) replaced with CaMKK…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha
Plasmid#33330DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
UseTagsFLAGExpressionMammalianMutationCaMKKbeta N-terminus (1-164) was fused to body fo…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKalpha N-terminus on CaMKKbeta
Plasmid#33329DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationCaMKKalpha N-terminus(1-128) was fused with CaMKK…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
Venus Cam KII alpha 1-315aa
Plasmid#29431DepositorInsertVenus CAM KII Alpha
UseTagsVenusExpressionMammalianMutationExpresses CamKIIa amino acids 1-315PromoterAvailable sinceJuly 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag
Plasmid#179719PurposeLentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag
Plasmid#179723PurposeLentiviral expression of lysosome-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO
Plasmid#128755PurposeExpresses CYRANO gRNA for CRISPRiDepositorInsertCYRANO sgRNA (OIP5-AS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GFP-IKBalpha-mut (super repressor)
Plasmid#15264DepositorInsertIKB alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationSerine 32 to Alanine, Serine 36 to AlaninePromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA Ef1alpha Puro-T2A-GFP
Plasmid#111596PurposesgRNA Ef1alpha Puro-T2A-GFPDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R]
Plasmid#177486PurposeMammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.DepositorInsertanti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)
Plasmid#188385PurposeLentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factorDepositorInsertPGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter luciferase delta CRE
Plasmid#8888DepositorInsertPGC-1 alpha promoter dCRE (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationSite-directed mutagenesis to remove the CREB bind…PromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV FLAG Rev-Erb alpha
Plasmid#22745DepositorInsertRev Erb alpha (NR1D1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)
Plasmid#225433PurposeTranscriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.DepositorInsert4xCBS:mDFR:Rep/RepA:Tnos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164
Plasmid#122966PurposeExpresses 3XFlag-tagged human CEP164 in mammalian cellsDepositorInsertCEP164 (CEP164 Human)
UseLentiviralTags3XFlagExpressionMutationPromoterEF1 alphaAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter luciferase delta MEF
Plasmid#8889DepositorInsertPGC-1 alpha promoter dMEF (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationSite-directed mutagenesis to remove the MEF2 bind…PromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204340PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-s/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
hum alpha ENAC promoter a25-a23
Plasmid#83427Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromoteralpha ENACAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-PKIalpha-IRES-nls-mRuby2
Plasmid#63059PurposeExpresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertPKIalpha (Pkia Synthetic, Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204341PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos
Plasmid#211840PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.DepositorInsertLRR-NEL
UseSynthetic BiologyTagsHA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
V309 - pDNR MCS SA lacZ alpha - Donor
Plasmid#11692DepositorTypeEmpty backboneUseCre/Lox; Donor vectorTagsExpressionMutationPromoterAvailable sinceMay 24, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211843PurposePlant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.DepositorInsertLRR-NEL
UseSynthetic BiologyTagsHA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204343PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S
Plasmid#211839PurposePlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stat1 alpha Y701F Flag pRc/CMV
Plasmid#8702DepositorInsertSTAT1 alpha Y701F (STAT1 Human)
UseTagsFlagExpressionMammalianMutationY701FPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV MP-135 mRFP-3MREs (AAV-alpha)
Plasmid#206929PurposeAlpha cell-specific gene expression from AAV in human pancreatic isletsDepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterMP-135Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control
Plasmid#128749PurposeExpresses control gRNADepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PBS/pU6- HIF1 alpha RNAi plasmid 1
Plasmid#21103DepositorInsertRNAi against hypoxia inducible factor 1alpha
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-VP64-ZsG
Plasmid#192667Purpose3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1DepositorInsertVP64-dCas9-VP64, ZsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha A-crystallin 1kb promoter/AcGFP
Plasmid#98096PurposeaA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha A-crystallin 1 kb fragment (-1028/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204342PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-s/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)
Plasmid#160633PurposeTU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)DepositorInsertPhiC31-RDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)
Plasmid#178851PurposeMammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
UseTagsExpressionMammalianMutationGαq: C9S/C10S/Q209L/L254APromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-p65-HSF1-Blast
Plasmid#192652Purpose3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance markerDepositorInsertscFv-p65-HSF1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry
Plasmid#136603PurposeEncodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).DepositorInsertFKBP-C-mSOG-J-alpha
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PBS/pU6-HIF1 alpha RNAi plasmid 2
Plasmid#21104DepositorInsertRNAi against hypoxia inducible factor 1alpha
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
V308 pDNR MCS lacZ alpha - Donor
Plasmid#11691DepositorTypeEmpty backboneUseCre/Lox; Donor vectorTagsExpressionMutationPromoterAvailable sinceMay 24, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L
Plasmid#122991PurposeExpresses Flag-tagged hRPGRIP1L in mammalian cellsDepositorInsertRPGRIP1L (RPGRIP1L Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1 alphaAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)
Plasmid#52216Purposemammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutationDepositorInsertHIF1alpha (401delta603) LCLL (HIF1A Human)
UseTagsHAExpressionMammalianMutationaa 401-603 deleted, removes the oxygen-dependent …PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta LIM
Plasmid#40933DepositorInsertEPLIN alpha (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationLIM domain deleted- amino acids 221-295PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir144 EF1Alpha-puro-T2A-BFP
Plasmid#164791PurposeExpress miR-144 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta C-term
Plasmid#40930DepositorInsertEPLIN alpha (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 296-600PromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
Plasmid#164800PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1DepositorInsertsERH CRISPRi gRNA1
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only