We narrowed to 7,456 results for: Lif;
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Plasmid#122562PurposeIntegration of Cre sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-Cre-SV40pA
UseCloningTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
P223
Plasmid#139720PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, CPHDepositorInsertpMOD_A'_pLHB1B1:NnCPH:tSlH4
UseLuciferaseTagsExpressionPlantMutationPromoterAtLHB1B1Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-miniTurbo
Plasmid#166567PurposeMultisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQDepositorInsertminiTurbo
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-BASU-NS
Plasmid#166565PurposeMultisite Gateway middle entry clone encoding BASU. Parton lab clone JRODepositorInsertBASU
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA (KmR)
Plasmid#102321Purposedrives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)DepositorInsertRpaA with p0050 promoter (+ kanamycin resistance casette)
UseCloning vectorTagsExpressionMutationPromoter-Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP38
Plasmid#65616PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edCit-attR1 and attR2-edCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP43
Plasmid#65618PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3
Plasmid#122092PurposeAAV plasmid for expressing rsCaMPARI in neurons, nucleus localizedDepositorInsertrsCaMPARI
UseAAVTagsNLS-His and mRuby3ExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-BFLF1
Plasmid#162626PurposeExpresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cellsDepositorInsertBFLF1
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLIP37
Plasmid#65505PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-Cer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn CCL20-pre-mGRASP
Plasmid#173117PurposeExpresses CCL20 sender construct in neuronsDepositorInsertCCL20-pre-mGRASP
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn Arrestin-TEVp-V5
Plasmid#173118PurposeExpresses arrestin receiver construct in neuronsDepositorInsertArrestin-TEVp-V5
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Paxillin LD
Plasmid#186150PurposeFor expressing recombinant paxillin variants in bacteria.DepositorInsertPXN (PXN Human)
UseTagsGSTExpressionBacterialMutationD5A; D146A; D232A; D267A; D301APromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Paxillin
Plasmid#186143PurposeFor expresseion of recombinant paxillin in bacteriaDepositorInsertPXN (PXN Human)
UseTagsGSTExpressionBacterialMutationPaxillin C1997A (silent mutation for cloning)PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3195
Plasmid#172695PurposePunc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)DepositorInsertPunc-25
UseTagsGFP 1-10ExpressionWormMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::RpaA D53A
Plasmid#105229Purposeinducible expression of unphosphorylatable variant of RpaA (D53A)DepositorInsertrpaA driven by Ptrc promoter
UseCyanobacteria cloning vectorTagsExpressionMutationD53 mutated to APromoterptrcAvailable sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Char-BFP2-TSERex
Plasmid#124792PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertChar-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromoterAtuMASAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TurboID-CaaX
Plasmid#163848PurposeIn vitro transcription of TurboID-CaaX. Parton lab clone JSUDepositorInsertTurboID
UseIn vitro transcription of rnaTagsCaaXExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w
Plasmid#162481PurposeA bicistronic construct - FloxGal80 and UAS-mCD8GFPDepositorInsertCre80Tom-GFP
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK33/intein-Cas9_S219-3XFLAG
Plasmid#133782PurposeExpresses human codon-optimized intein-Cas9DepositorInsertHuman codon-optimized intein-Cas9
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx4
Plasmid#104474PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
UseTagsFKBPx4-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP154
Plasmid#106360PurposeA vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LexAop2->Bxb1.STOP>myr::4xSNAPf
Plasmid#87640PurposeFor creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPfDepositorInsertmyr::4xSNAPf
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP30
Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
tagRFP-GPHN.FingR-PhoCl
Plasmid#236183PurposeHalf of photoactivatable GFE3. When co-expressed with PBP-E3-HA (Plasmid #236182), ablates inhibitory synapses after exposure to 400 nm light.DepositorInserttagRFP-GPHN.FingR-PhoCl
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-PFE3-P2A-GFP
Plasmid#236180PurposeAAV version of Tet inducible PFE3 for ablating excitatory synapses.DepositorInsertPFE3-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
RNF168 C-terminal sgRNA
Plasmid#207100PurposepX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.DepositorInsertGAGATGCACAAAGTAAGGCC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
RIF C-terminal sgRNA
Plasmid#207096PurposepX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.DepositorInsertAATACTAAATAGAATTTTCA
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
REV7 C-terminal sgRNA
Plasmid#207095PurposepX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.DepositorInsertGCTCATAAAGGCAGCTGAGG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-BoxB-petracrRNA
Plasmid#207625PurposeExpresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)DepositorInsertBoxB-petracrRNA
UseSynthetic BiologyTagsExpressionMutationPromoterhU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pDestTol2-QUAS:GFP-CAAX-he1.1:YFP
Plasmid#184816PurposeUsed to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic lineDepositorInsertQUAS:GFP-CAAX-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsGFP-CAAXExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDR274-gsc2-sgRNA
Plasmid#184818PurposeUsed to synthesize gRNA targeting exon 2 of the gsc2 geneDepositorInsertgsc2-sgRNA
UseCRISPR; Template for sgrna synthesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDestTol2-QUAS:NLS-mApple-he1.1:CFP
Plasmid#184814PurposeUsed to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic lineDepositorInsertQUAS:NLS-mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-mAppleExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-GFP-he1.1:CFP
Plasmid#184815PurposeUsed to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic lineDepositorInsertQUAS:NLS-GFP-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-GFPExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHLD3 N-terminal sgRNA
Plasmid#207094PurposepX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.DepositorInsertTTACTGCAGAATGACTACAG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBS1 C-terminal sgRNA
Plasmid#207092PurposepX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.DepositorInsertTAAAAAGGAGAAGATAACTG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169 C-terminal sgRNA
Plasmid#207099PurposepX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.DepositorInsertACACTTCATTAGGTGCTACT
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHLD1 N-terminal sgRNA
Plasmid#207101PurposepX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.DepositorInsertATGGCAGGACTATGGCAGCC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATM C-terminal sgRNA
Plasmid#207097PurposepX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.DepositorInsertTTTCTAAAGGCTGAATGAAA
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHLD3 KO sgRNA #1
Plasmid#207105PurposepX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..DepositorInsertCGCTATCAAGATTTATACCT
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorInsertHistone H3 (HIST2H3PS2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHLD2 N-terminal sgRNA
Plasmid#207098PurposepX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.DepositorInsertTTTTTATCAGAAATCATGAG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only