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Plasmid#190250PurposeExpression SUMO7-SIM6 in yeast cellsDepositorInsert7SUMO-6SIM
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_128-372 (MCSI)_E
Plasmid#146174PurposeBacterial Expression of DmDcp1_128-372DepositorInsertDmDcp1_128-372 (Dcp1 Fly)
UseTagsExpressionBacterialMutation3 amino acid del (PQQ 318-320)PromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)_G
Plasmid#146402PurposeBacterial Expression of DmDCP1_328-366DepositorInsertDmDCP1_328-366 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_1-127 (MCSII)_E
Plasmid#146173PurposeBacterial Expression of DmDCP1_1-127DepositorInsertDmDCP1_1-127 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S7-S83C/Δ157-178
Plasmid#128592PurposeExpresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coliDepositorInsertS7 E.coli ribosomal protein (rpsG E.coli)
UseTagsExpressionBacterialMutationS83C, Δ157-178PromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetMP-CeSmg7_1-395-G80E_S
Plasmid#147476PurposeBacterial Expression of CeSmg7_1-395-G80EDepositorInsertCeSmg7_1-395-G80E (smg-7 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsPatL1_1-111 (MCSII)_B
Plasmid#145968PurposeBacterial Expression of HsPatL1_1-111DepositorInsertHsPatL1_1-111 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsPatL1_1-173 (MCSII)_B
Plasmid#145969PurposeBacterial Expression of HsPatL1_1-173DepositorInsertHsPatL1_1-173 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsPatL1_1-398 (MCSII)_B
Plasmid#145970PurposeBacterial Expression of HsPatL1_1-398DepositorInsertHsPatL1_1-398 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK_296-472(MCSI)_B
Plasmid#145971PurposeBacterial Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-59_B
Plasmid#145973PurposeBacterial Expression of HsPatL1_1-59DepositorInsertHsPatL1_1-59 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-173_B
Plasmid#145975PurposeBacterial Expression of HsPatL1_1-173DepositorInsertHsPatL1_1-173 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-398_B
Plasmid#145976PurposeBacterial Expression of HsPatL1_1-398DepositorInsertHsPatL1_1-398 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu R126A
Plasmid#129376Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu R126A
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu R126I
Plasmid#129377Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu R126I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu A81I
Plasmid#129352Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu A81I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu D89A
Plasmid#129354Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu D89A
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu V90D
Plasmid#129355Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu V90D
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu A81E
Plasmid#129356Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu A81E
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu I88D
Plasmid#129357Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu I88D
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-111_B
Plasmid#145974PurposeBacterial Expression of HsPatL1_1-111DepositorInsertHsPatL1_1-111 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1-ATG7-3'UTR
Plasmid#166847PurposeExpresses Atg1 with ATG7 3'UTR in yeasts.DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATG1 PromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A-GFP
Plasmid#166837PurposeExpresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationG2APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)HIB
Plasmid#134675PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. ColiDepositorInsertsTglA(C51)
TglH
TglI
TglB
UseTagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-3P-ECM33_intron
Plasmid#127601Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 3′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5M-ECM33_intron
Plasmid#127600Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5P-ECM33_intron
Plasmid#127599Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-ECM33_intron
Plasmid#127598Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-no_intron
Plasmid#127597Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoterDepositorInsert1xFLAG-MCP expression cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1(mut)(406)
Plasmid#122069PurposeExpressing a mutant ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationCodon changes without changing the amino acid seq…PromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG7p-ATG1(406)
Plasmid#122071PurposeExpressing ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationThe native promoter was replaced by ATG7 promoter…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9-GFP T119D
Plasmid#114668PurposeExpresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tagDepositorInsertATG9
UseTagsGFPExpressionYeastMutationPromoterATG9Available sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D/D450G
Plasmid#87287PurposeEncodes Hsh155 N295D D450G for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN295D & D450GPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/H331D
Plasmid#87282PurposeEncodes Hsh155 R294L H331D for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationR294L & H331DPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D/H331D
Plasmid#87285PurposeEncodes Hsh155 N295D H331D for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN295D & H331DPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D/K335E
Plasmid#87286PurposeEncodes Hsh155 N295D K335E for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN295D & K335EPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/D450G
Plasmid#87284PurposeEncodes Hsh155 R294L D450G for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationR294L & D450GPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/K335E
Plasmid#87283PurposeEncodes Hsh155 R294L K335E for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationR294L & K335EPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(E224Q, GiVL-2)
Plasmid#32035DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationE224Q and N195-M215 replaced by P697-D714 of GiDi…PromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(E147Q/D151N)
Plasmid#32024DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationE147Q/D151NPromoterT7Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 L94A
Plasmid#18691DepositorInsertRnq1 L94A
UseTagsExpressionYeastMutationLeucine 94 mutated to Alanine (L94A)PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
7861 pRS305-pUG72-HSC82out
Plasmid#10899DepositorInsertHSC82 URA3 (HSC82 Budding Yeast)
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-AqLumSynth –Sapphire
Plasmid#116931PurposeYeast integrative vector for the exression of the 20nm GEM under the HIS3 promoterDepositorInsertlumazine synthase
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)
Plasmid#193049PurposeEncodes G-418 resistanceDepositorInsertprsp-27::NeoR::unc-54 3’ UTR
UseTagsExpressionWormMutationPromoterprsp-27Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Puro-Ak-ACE2-TMPRSS2
Plasmid#154987PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-6xHis mAb
Plasmid#214952PurposeBacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.DepositorInsertsVNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain
UseTagsVNp6ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only