We narrowed to 3,021 results for: COB
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Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9ExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available SinceSept. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
HiFi Cas9
Plasmid#188490PurposeExpresses FLAG tagged HiFi Cas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianMutationR691APromoterEF1aAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW2082
Plasmid#236576PurposeISYmu1 PDS3 gRNA12 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
ExpressionPlantAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD42-BtuCEDFB
Plasmid#237541Purposeplasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteinsDepositorInsertsbtuB
btuF
btuCED
ExpressionBacterialPromoterpBADAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 nog
Plasmid#120251PurposeCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
plRL19
Plasmid#163634PurposeL5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).DepositorInsertL5 Integrase
ExpressionBacterialPromoterMycobacterial Optimized Promoter (MOP) and Tet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-eGFP-W
Plasmid#126686PurposeConstitutive, ubiquitous GFP expression.DepositorInserteGFP
UseLentiviralPromoterEF1aLAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW2658
Plasmid#252576PurposeTRV2 multiplex vector expressing Ymu1-WFR, AtCHLl1 gRNA4 and AtPDS3 gRNA12DepositorInsertYmu1-WFR with AtCHLl1 gRNA4 and AtPDS3 gRNA12
UseCRISPRExpressionPlantAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 nog
Plasmid#115480PurposeCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW2657
Plasmid#252577PurposeTRV2 multiplex vector expressing Ymu1-WFR, AtPDS3 gRNA12 and AtCHLl1 gRNA4DepositorInsertYmu1-WFR with AtPDS3 gRNA 12 and AtCHLl1 gRNA4
UseCRISPRExpressionPlantAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSUM-GroEL-gfp
Plasmid#109286PurposeExpresses gfp in mycobacteriaDepositorInsertGroEL-GFP
ExpressionBacterialMutationGFP modified for expression in prokaryotes; inclu…PromoterGroELAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW2066
Plasmid#236575PurposeISYmu1 PDS3 gRNA2 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
ExpressionPlantAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 KRAB
Plasmid#188499PurposeExpresses FLAG tagged SpdCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A/H840APromoterEF1aAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneTags3xFLAGExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRExpressionBacterialPromoterTetOAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-FGE
Plasmid#75147PurposeExpression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.DepositorInsertFGE
TagsHistidine tag and ThrombinExpressionBacterialMutationN/APromoterT7Available SinceJuly 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
TagsmCherryExpressionBacterialAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only