We narrowed to 7,568 results for: Lif

1. pHR-mCherry-Emi1_ARHGAP5 UTRs

   Plasmid

   #67927

   Purpose

   Expresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cells

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   mCherry-Emi1 (FBXO5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW4.28

   Plasmid

   #62878

   Purpose

   Bacterial expression of MBP-MEG-3 T541A S582A T605A

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   MBP-MEG-3 T541A S582A T605A
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   T541A S582A T605A

   Promoter

   Available since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW3.31

   Plasmid

   #62865

   Purpose

   Yeast two-hybrid bridge vector expressing PAA-1

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   PAA-1/Y3H
   

   Use

   Two-hybrid bridge vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Ubl4A K48R

   Plasmid

   #61809

   Purpose

   expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   UBL4A (UBL4A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K48R

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pLX-TRE-dCas9-KRAB-MeCP2-BSD

   Plasmid

   #140690

   Purpose

   Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   Cas9m4-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. keratin 8 (krt8)-H2B-RFP

   Plasmid

   #105955

   Purpose

   transgenesis

   Depositor

   Caren Norden

   Article

   Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

   Insert

   krt8 (KRT8 Human)
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-Amp_CRISPIE donor B5

   Plasmid

   #172846

   Purpose

   CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_VCL sgRNA / hSpCas9

   Plasmid

   #172837

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_TOMM20 sgRNA / hSpCas9

   Plasmid

   #172836

   Purpose

   Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid

    #172834

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_CALR sgRNA / hSpCas9

    Plasmid

    #172838

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer20 Cyclin E1

    Plasmid

    #109348

    Purpose

    virus packaged, doxycyline inducible expression of Cyclin E1

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    Cyclin E1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid

    #163928

    Purpose

    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330 _CRISPIE donor B8

    Plasmid

    #172849

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330 _CRISPIE donor B7

    Plasmid

    #172848

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330 _CRISPIE donor B9

    Plasmid

    #172850

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-OSF-BECN1

    Plasmid

    #99328

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    Beclin 1 (BECN1 Human)
    

    Use

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-OSF-VPS34

    Plasmid

    #99327

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    VPS34 (PIK3C3 Human)
    

    Use

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR

    Plasmid

    #67931

    Purpose

    Translational reporter - ARHGAP5 UTRs

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    sfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Nrxn3α-AS4-

    Plasmid

    #184332

    Purpose

    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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