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We narrowed to 2,422 results for: dcas9

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Showing: 401 - 420 of 2422 results

  1. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non-targeting sgRNA human (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71830-plasmid-map-sequence-id-131025

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  3. pCMV dCas9-MC (eGFP)

    Plasmid
    #89931
    Purpose
    Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Publication
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-MC
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deactivated Cas9, fragment of M.SssI (residues 27…
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89931-plasmid-map-sequence-id-177995

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  7. pRTS-GBP-dCas9-mRFP

    Plasmid
    #102934
    Purpose
    Tetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)
    Depositor
    Sebastian Bultmann
    Publication
    Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    GBP (GFP binding protein and mRFP
    Expression
    Mammalian
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  19. M3-M14-dCas9

    Plasmid
    #134779
    Purpose
    Expression of fusion protein M3-M14-dCas9 in Mammalian cells
    Depositor
    Shu-Bing Qian
    Publication
    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 S. pyogenes, Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134779-plasmid-map-sequence-id-267134

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Showing: 401 - 420 of 2422 results