We narrowed to 7,218 results for: ef1a;

1. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

   Plasmid

   #155306

   Purpose

   Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-V5-Bet1

   Plasmid

   #120243

   Purpose

   Expresses V5-tagged Bet1 in lentiviral vector

   Depositor

   Larry Gerace

   Insert

   Bet1 (Bet1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1a-hMLH1dn (og codon)

   Plasmid

   #174823

   Purpose

   Mammalian expression of human MLH1dn (original codon usage)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (original codon usage) (MLH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-EF1aL-dsRed-W

   Plasmid

   #195863

   Purpose

   lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

   Depositor

   Darrell Kotton

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   dsRed

   Expression

   Mutation

   Promoter

   EF1aL

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-EF1aL-mCherry-W

   Plasmid

   #126703

   Purpose

   Constitutive, ubiquitous mCherry expression.

   Depositor

   Darrell Kotton

   Article

   McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1aL

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-LIC-V5

   Plasmid

   #120248

   Purpose

   For LIC cloning with C-terminal V5 tag

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH21-Tier1-PhEF1a

   Plasmid

   #169561

   Purpose

   Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112924

   Purpose

   piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Piggybac transposon vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1a-Usp9x_CD-mCherry

    Plasmid

    #170380

    Purpose

    Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

    Depositor

    Miguel Ramalho-Santos

    Article

    Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

    Insert

    Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
    

    Use

    Tags

    mCherry-3xFlag

    Expression

    Mammalian

    Mutation

    Catalytic domain

    Promoter

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1A-CCNE2

    Plasmid

    #162554

    Purpose

    Overexpression of CCNE2

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE2 (CCNE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mc2 mNeon EF1a

    Plasmid

    #206224

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV-EF1a-mCherryCXCR4-W

    Plasmid

    #200102

    Purpose

    Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    silent mutations (c.180C>T, 438G>A, 495C>…

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-sCre

    Plasmid

    #55635

    Purpose

    Expresses sCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-NucGFP

    Plasmid

    #184706

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_EF1a-->sGFP_PGK-->puroR

    Plasmid

    #162156

    Purpose

    Lentiviral vector for constitutive expression of GFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-V5-LIC

    Plasmid

    #120247

    Purpose

    For LIC cloning with N-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1a_FL MUT TET1

    Plasmid

    #124081

    Purpose

    Expression of catalytic inactive form of full-length TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - full length, mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112922

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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