We narrowed to 24,612 results for: Nes

1. pMax_GFP_C289T

   Plasmid

   #177826

   Purpose

   Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP_C289T
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C289T

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHLuc (Antares-SEPLuc C1A4E+6)

   Plasmid

   #141089

   Purpose

   final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. hCMV-IE1:GrRenilla

   Plasmid

   #118067

   Purpose

   Transcriptional unit for constitutive expression of GrRenilla in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:GreenRenilla:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-Bnip3FL

   Plasmid

   #100796

   Purpose

   Mammalian expression of Bnip3 full length splice variant

   Depositor

   Joseph Gordon

   Article

   Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

   Insert

   mus Bnip3 (Bnip3 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5xNF-kb_RE

   Plasmid

   #118050

   Purpose

   GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   5 copies of the NF-kb DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1 3xFlag-CREB

   Plasmid

   #82721

   Purpose

   Expresses human CREB

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   CREB (CREB1 Human)
   

   Use

   Tags

   3XFLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX302-DUSP19-V5 puro

   Plasmid

   #87772

   Purpose

   Expresses V5 tagged human DUSP19

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   DUSP19 (DUSP19 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMax_BFP_A191G

   Plasmid

   #177823

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP_A191G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A191G

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304-CAR-V5

   Plasmid

   #82723

   Purpose

   Expresses V5 tagged coxsackievirus and adenovirus receptor (CAR).

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   coxsackievirus and adenovirus receptor (CXADR Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)

    Plasmid

    #179078

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid

    #100025

    Purpose

    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Use

    Tags

    V5 Epitope Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDFDuet-MKK6-EE

    Plasmid

    #82718

    Purpose

    Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

13. AAV-CREB

    Plasmid

    #68550

    Purpose

    AAV expression of CREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    CREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. tet_pLKO.1_puro_shLuc

    Plasmid

    #136587

    Purpose

    Expresses an inducible short hairpin targeting firefly luciferase sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB2904(XI-3 MarkerFree)

    Plasmid

    #73276

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. tet-pLKO shGATA6 puro

    Plasmid

    #72615

    Purpose

    Expresses an inducible short hairpin targeting human GATA6 sequence

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    short hairpin GATA binding protein 6
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tight

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK TT 3xFLAG SMAD4 neo

    Plasmid

    #83273

    Purpose

    Lentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. hCMV-IE1:ELuc

    Plasmid

    #118062

    Purpose

    Transcriptional unit for constitutive expression of ELuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:ELuc:bGH
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PatoL-OG241

    Plasmid

    #72841

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PatoL

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRedF

    Plasmid

    #118057

    Purpose

    GBPart - RedF Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Red Firefly Luciferase CDS
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Red mutant S286Y

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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