We narrowed to 14,467 results for: cas9 genes

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MSP977

Plasmid

#65774

Purpose

Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E mutation in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGE006

Plasmid

#108722

Purpose

Cas9 expression in M.polymorpha

Depositor

Takayuki Kohchi, Shigeo Sugano

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Insert

Atco-Cas9-Pea3ter

Use

CRISPR

Expression

Plant

Promoter

MpEFpro

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTL-Bag3-gRNA3

Plasmid

#196254

Purpose

Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag2-gRNA2

Plasmid

#196252

Purpose

Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag1-gRNA1

Plasmid

#196251

Purpose

Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag4-gRNA4

Plasmid

#196255

Purpose

Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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M-ST1-VPR

Plasmid

#68498

Purpose

nuclease competent ST1-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

ST-Cas9-VPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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SpG-IP

Plasmid

#242073

Purpose

Expresses Cas9-SpG in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpG

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-IP

Plasmid

#235994

Purpose

Expresses Cas9-SpRY in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa VP64 Hbb

Plasmid

#99693

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)

Use

AAV and CRISPR

Tags

VP64

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMLS338

Plasmid

#73719

Purpose

SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Unc-32::GFP(Cbr-unc-119)

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pPN10-gDM1d

Plasmid

#114387

Purpose

pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gDM1d

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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pPN10-gCTG

Plasmid

#114385

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCTG

Use

CRISPR

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDB4279

Plasmid

#98698

Purpose

A control plasmid containing the intact bsdMX marker

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPN10-gCAG

Plasmid

#114384

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCAG

Use

CRISPR

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRB1081

Plasmid

#71479

Purpose

Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRB1084

Plasmid

#71516

Purpose

Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLV.5’-LMNA-eGFP-KI

Plasmid

#170545

Purpose

Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for LMNA knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’VIM-eGFP-KI

Plasmid

#170547

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for VIM knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSC51

Plasmid

#104825

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.12g075700, Glyma.11g145900

Use

CRISPR

Expression

Plant

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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