We narrowed to 6,668 results for: PROC

1. AAV-short_BrokenHeart (no BC)

   Plasmid

   #86950

   Purpose

   AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   Brokenheart construct
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRLuc-CoV2-delStem1

   Plasmid

   #177616

   Purpose

   Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   3-nucleotide (UAC) deletion in Stem 1

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRLuc-CoV2-FLucStop

   Plasmid

   #177617

   Purpose

   Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   In-frame stop codon added between FLuc and FSE

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCerulean-proα1(I)

   Plasmid

   #119842

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT

   Plasmid

   #60419

   Purpose

   Mammalian Expression of ITSN DH/PH-tgRFPt-Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   hITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   tgRFPt

   Expression

   Mammalian

   Mutation

   hITSN 1159-1509 // SSPB Y11K and A15E

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG106 Lyn11-FAST

   Plasmid

   #130721

   Purpose

   Expresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   lyn11-FAST
   

   Tags

   myc-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER12 (miR-RUL)

   Plasmid

   #46935

   Purpose

   Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

   Depositor

   Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Insert

   PheS Gly294
   

   Use

   Lentiviral

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EFS-DIO-GRAB_Ado1.0med

   Plasmid

   #153292

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-PHFF

   Plasmid

   #100280

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRY2-INPP5E

    Plasmid

    #79562

    Purpose

    expression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    C-terminal region, aa 214_644, C641A mutation to …

    Promoter

    CMV

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. CRY2-INPP5E(D556A)

    Plasmid

    #79564

    Purpose

    expression of CRY2PHR fused to catalytically dead INPP5E

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    C-terminal region, aa 214_644, C641A mutation to …

    Promoter

    CMV

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

12. mCherry-CRY2-INPP5E

    Plasmid

    #79561

    Purpose

    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    C-terminal region, aa 214_644, C641A mutation to …

    Promoter

    CMV

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET28_6xHis-PARP1-CAT-L713F

    Plasmid

    #173944

    Purpose

    Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CAT-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. alpha actinin delta ABD-GFP

    Plasmid

    #66935

    Purpose

    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

    Depositor

    Pere Roca-Cusachs, Michael Sheetz

    Article

    Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Alpha-actinin 1 (ACTN1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    actin binding domain (amino acids 30–253) is dele…

    Promoter

    CMV

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pENTR1A mKate2-hu_ncOGT WT

    Plasmid

    #154282

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    WT OGT

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX_305-N-dTAG_OGT

    Plasmid

    #155196

    Purpose

    N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    lacking N-terminal Met

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR-Flag-IKKalpha-KM

    Plasmid

    #15468

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha-KM (Chuk Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Lysine at 44 to Alanine

    Available Since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid

    #204215

    Purpose

    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    Cas13bt3 and U6.SapI sgRNA cloning site
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimisation by GenScript tool

    Promoter

    CMV/U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG109 H2B-FAST

    Plasmid

    #130722

    Purpose

    Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    H2B-FAST
    

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mApple-proα2(I)-L

    Plasmid

    #120161

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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