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pBSV2G_P0826-BB0323-mCherryBb

Plasmid

#118244

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

BB0323-mCherry translational fusion under the control of the bb0826 promoter

Tags

mCherry

Expression

Bacterial

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-VPS28

Plasmid

#34803

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

vacuolar protein sorting 28 (yeast) (VPS28 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pXcX-PRS-EGFP

Plasmid

#25865

Depositor

Sergey Kasparov

Article

Teschemacher et al Hypertension. 2008 Aug . 52(2):351-8.

Insert

PRS driven enhanced green fluorescent protein

Expression

Mammalian

Available Since

Aug. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v3

Plasmid

#169464

Purpose

Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-TPR

Plasmid

#23519

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TPR (TPR Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
GFP-Myo 9b

Plasmid

#134907

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Non-specific gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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sh-RNA hULK2

Plasmid

#31970

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Ec-Kbp-D9N

Plasmid

#178011

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Ec-Kbp-D9N

Expression

Bacterial

Promoter

araB

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-CRM197-4xDQNAT

Plasmid

#128403

Purpose

Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp16-HF

Plasmid

#157725

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGene-TAP-GBP1

Plasmid

#138087

Purpose

Plasmid with TAP-GBP1 ORF

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Insert

human GBP1 (GBP1 Human)

Tags

Tandem affinity purification (TAP) tag

Expression

Mammalian

Promoter

Gal4-E1b

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-CRY2-uTEV1Δ(220-242)

Plasmid

#135452

Purpose

Yeast expression of uTEV1Δ fused to CRY2 and mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-CRY2-TEV1Δ(220-242)

Tags

mCherry

Expression

Yeast

Promoter

Gal

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

Plasmid

#99224

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.2

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLostfoX-Kan

Plasmid

#99865

Purpose

TfoX induction vector for chitin-pathway transformation (KanR)

Depositor

Mark Mandel

Article

Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

Insert

tfoXVF

Use

V. fischeri suicide cloning vector, cmr, tcr

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shOrai1

Plasmid

#89933

Purpose

Knockdown human Orai1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

Orai1 shRNA (ORAI1 Human)

Use

Retroviral

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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