We narrowed to 9,297 results for: ef1

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P2A-NLS-tdtomato-EPG backbone

Plasmid

#215548

Purpose

An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Type

Empty backbone

Use

CRISPR

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD

Plasmid

#170124

Purpose

Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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P2A-mCherry-EhT backbone

Plasmid

#215549

Purpose

An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Type

Empty backbone

Use

CRISPR

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Sico-Red

Plasmid

#84882

Purpose

Cre-inducible shRNA

Depositor

Eun Mi Hwang

Article

Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi

Insert

Sico-Red

Use

AAV

Tags

mCherry

Promoter

EF1a and CMV

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-RT006-GCN4-HaloPM

Plasmid

#163386

Purpose

To express 4X sGCN4 module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG

Plasmid

#175501

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB408

Plasmid

#68673

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pILFPQ13BE5

Plasmid

#223836

Purpose

Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>ApacH>StayGold2

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMpGWB236

Plasmid

#68627

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLX307 hcRed1

Plasmid

#117732

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pWXLd-RIEP-GpNLuc

Plasmid

#135935

Purpose

Expresses the GpNLuc LumiFluor in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pWXLd-RIEP-CeNLuc

Plasmid

#135934

Purpose

Expresses the CeNLuc LumiFluor in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-DIO-CB2-EGFP

Plasmid

#91946

Purpose

Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Inserts

EGFP
type-2 cannabinoid receptor

Use

AAV

Tags

FLAG and None

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLX311 hcRed1

Plasmid

#117733

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-tTAc

Plasmid

#172125

Purpose

Expression of InteinC-tTAC in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, tTAc

Use

AAV

Promoter

CaMKII

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_213

Plasmid

#133456

Purpose

H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

Control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB336

Plasmid

#68664

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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