We narrowed to 11,275 results for: Vars;

1. pOPINVHH_A10-5_His

   Plasmid

   #201720

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_A10-5 (S lama glama (llama))
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINVHH_B5-5_His

   Plasmid

   #201721

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH B5-5 (S lama glama (llama))
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-enCas12a-A3A-N57G

   Plasmid

   #193651

   Purpose

   Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLb-enCas12a-hA3A-N57G, EGFP
   

   Expression

   Mammalian

   Mutation

   dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

   Plasmid

   #201576

   Purpose

   Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutations S172A, M173A and V195A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Stx5L(m55V)-GFP

   Plasmid

   #209845

   Purpose

   Expresses Stx5 M55V mutant fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   STX5 (STX5 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Methionine 55 to Valine, causes loss of S…

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ERAP1-rs27895(C)

   Plasmid

   #206142

   Purpose

   Expresses the rs27895-C allele of ERAP1, myc-tagged.

   Depositor

   Dennis Ko

   Article

   Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

   Insert

   ERAP1 (ERAP1 Human)
   

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. K365-iLid

   Plasmid

   #188456

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Zvonimir Dogic

   Article

   Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

   Insert

   Kinesin 1-365 (Khc Fly)
   

   Tags

   6xHis and iLid

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-365

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. K365-micro

   Plasmid

   #188457

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Zvonimir Dogic

   Article

   Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

   Insert

   Kinesin 1-365 (Khc Fly)
   

   Tags

   6xHis-MBP-TEV and SspB micro

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-365

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBT-RP8.3

    Plasmid

    #189848

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Promoter

    beta Actin

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

    Plasmid

    #148520

    Purpose

    Mammalian Expression of HseIF4G1-L640AV646A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-L640AV646A (EIF4G1 Human)
    

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

    Plasmid

    #148521

    Purpose

    Mammalian Expression of HseIF4G1-LIVVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-LIVVtoAAAA (EIF4G1 Human)
    

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

    Plasmid

    #148522

    Purpose

    Mammalian Expression of HseIF4G1-Y619AL624AL625A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-Y619AL624AL625A (EIF4G1 Human)
    

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. RAT +D122H

    Plasmid

    #187426

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Mutation

    changed aspartic acid at 129 for histidine

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. RAT +R111E

    Plasmid

    #187425

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Mutation

    changed arginine at 118 to glutamic acid

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. IL2-mCherry-rGBD in pmCherryN1

    Plasmid

    #187286

    Purpose

    Express IL2-Rhotekin GTPase-binding domain-mCherry

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Human, Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-SopB(172-554)

    Plasmid

    #183664

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 172-554

    Promoter

    CMV

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-SopB(211-554)

    Plasmid

    #183665

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 211-554

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

    Plasmid

    #165083

    Purpose

    gRNA 2 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

    Plasmid

    #165085

    Purpose

    gRNA 1 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

    Plasmid

    #165086

    Purpose

    gRNA 2 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

    Plasmid

    #169468

    Purpose

    Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

    Plasmid

    #169466

    Purpose

    Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSico_U6-PGC1a1 sgRNA

    Plasmid

    #165425

    Purpose

    Expression of gRNA against human PGC-1a variant 1

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    gRNA against PGC-1a variant 1 (PPARGC1A Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

26. pCMV ShadowY-AURKA-mTurq2

    Plasmid

    #157770

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPGK-T7/2-CD44v2-10 (-cyt)

    Plasmid

    #137825

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region - N213S on the CD44 re…

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA 3.1 mitoAURKA Lys162Met

    Plasmid

    #157752

    Purpose

    Expression of kinase-dead AURKA K62M in mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP N1 AURKA 1-110

    Plasmid

    #157755

    Purpose

    Expression of AURKA Nter (aa1-110):GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The first 110 amino acids of AURKA were cloned

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP N1 AURKA 1-30

    Plasmid

    #157756

    Purpose

    Expression of AURKA Nter (aa1-30):GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The first 30 amino acids of AURKA were cloned

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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