We narrowed to 20,285 results for: ACE;

1. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-HA GCNF

   Plasmid

   #16336

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

   Insert

   GCNF (Nr6a1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS4

   Plasmid

   #55751

   Purpose

   Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Residues 1-59 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rap-MESA-20-M-Gal4

   Plasmid

   #84554

   Purpose

   MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   Rap-MESA-FRB-20-M-Gal4
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hADAM10 siRNA pSIREN-Retro-Q

   Plasmid

   #19140

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM10 siRNA
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

6. HA-MBP-TEVpCS-ADGRF4 CTF-FLAG

   Plasmid

   #217697

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRF4 (ADGRF4 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-384

   Promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

   Plasmid

   #217689

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE1 (EMR1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-584

   Promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EFS eGFP hPGK BSD

   Plasmid

   #188520

   Purpose

   Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CTAG_mScarlet-I

   Plasmid

   #136118

   Purpose

   FP for C-term fusion with CDS12

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CTAG-mScarletI(noATG)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. FabF (KASII) pet28 N-terminal His Tag

    Plasmid

    #75017

    Purpose

    Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    FabF (fabF E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pET22-HT/prsA

    Plasmid

    #73624

    Purpose

    Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC-CRISPR

    Plasmid

    #100008

    Purpose

    Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG

    Plasmid

    #167477

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

    Plasmid

    #167476

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTet-SNAP-HCA_sensor

    Plasmid

    #127182

    Purpose

    Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

    Depositor

    Kai Johnsson

    Article

    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

    Insert

    SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
    

    Use

    Tags

    HisTag and StrepTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNOC-CRISPR-HA

    Plasmid

    #99370

    Purpose

    Expresses Cas9-HA and sgRNA. Hygromycin resistance.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-HA

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BC-A1-002

    Plasmid

    #78689

    Purpose

    A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCK671.306

    Plasmid

    #192641

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZA-GFP-R5

    Plasmid

    #106256

    Purpose

    Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide

    Depositor

    Urartu Seker

    Article

    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Added silaffin R5 peptide to C terminus of GFP af…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

    Plasmid

    #70211

    Purpose

    1 Part of 4-part switch

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deCFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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