We narrowed to 10,102 results for: otos

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pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

Plasmid

#117656

Purpose

Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Inserts

sfCherry2(1-10)
sfCherry2(11)-SpyCatcher002

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAXipi

Plasmid

#53287

Purpose

Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-B-GECO1

Plasmid

#32448

Purpose

Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

CMV

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-zeo_3xHA-TurboID-NLS

Plasmid

#200022

Purpose

For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

3xHA-TurboID-NLS

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3Gs

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.0

Plasmid

#32447

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-iGABASnFR2n-WPRE

Plasmid

#218875

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

Synapsin

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMK198 (GAL-OsTIR1)

Plasmid

#140655

Purpose

GAL-OsTIR1(WT)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209661

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

GFAP

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep

Plasmid

#113883

Purpose

Mammalian (inducible) protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBADmod1-linker2-gamS

Plasmid

#45833

Depositor

Richard Murray, Vincent Noireaux

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

gamS

Use

Synthetic Biology

Tags

Histag

Expression

Bacterial

Promoter

pBAD

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-1P-Kv-WPRE

Plasmid

#202612

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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PS Intein/C1A Bid

Plasmid

#242025

Purpose

PS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein/C1A Bid

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNRDc-V5

Plasmid

#51297

Purpose

expression of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-EGFP-Rac1-wt

Plasmid

#12980

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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