We narrowed to 21,845 results for: Tes

1. pRK-flag-USP19 1-1290

   Plasmid

   #78579

   Purpose

   Expression flag-USP19 1-1290 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 1-1290 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 1291 residue to stop condon

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. SARS-CoV-2_N_deltalinker

   Plasmid

   #157870

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the linker

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaliner

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-PpU6P-sgRNA-L1L2

   Plasmid

   #113735

   Purpose

   Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pvimentin-miRFP713

   Plasmid

   #136569

   Purpose

   Vimentin labeling with near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   vimentin-miRFP713
   

   Use

   Tags

   miRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG1.1-TSSK2/3xFLAG

   Plasmid

   #199625

   Purpose

   Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Tssk2 mRNA (Tssk2 Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-U6a-U6c

   Plasmid

   #107599

   Purpose

   An entry vector with U6a and U6c promoter driving guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Inserts

   U6a promoter
   U6c promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAGhP14

   Plasmid

   #84362

   Purpose

   express FLAG-HA tagged human PRDM14

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   silent mutations at coding silent 1068CtoT, 1629T…

   Promoter

   CAG

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5/FRT/TO/intron/GFP

   Plasmid

   #113547

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-MT-mitfa

   Plasmid

   #81231

   Purpose

   expression of zebrafish mitfa

   Depositor

   David Raible, James Lister

   Article

   Lister et al Development. 1999 Sep;126(17):3757-67.

   Insert

   mitfa (mitfa Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   6 myc tags

   Expression

   Mammalian

   Mutation

   contains amino acids 5-412

   Promoter

   sCMV IE94 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

11. 9xHRE-MinTK-CRE

    Plasmid

    #141146

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    9xHRE-MinTK-CRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    reverse transcriptase D185K/D186L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TAL-L1-1.3-Ctrl

    Plasmid

    #102355

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pErbB2-ECFP

    Plasmid

    #66945

    Purpose

    Mammalian expression of rat ErbB2 tagged with ECFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pSin wPGK-Cre

    Plasmid

    #101242

    Purpose

    low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    Cre recombinase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    see attached file

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZLneo Flag mPTIP

    Plasmid

    #15558

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PTIP (Paxip1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pLK69

    Plasmid

    #37258

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    vang-like 2 (VANGL2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    lacks the 4 C-term aa

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pSM_ser-2prom3p::myr-mCherry

    Plasmid

    #164223

    Purpose

    Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

    Depositor

    Yan Zou

    Article

    Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

    Insert

    myr-mCherry
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    ser-2promo3

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

    Plasmid

    #31477

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 T2A mTagBFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y + S111Y on I-AniI = Y2 "reo" (res…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETx-SR6c3

    Plasmid

    #176211

    Purpose

    Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    SR6c3
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR223-BcLF1

    Plasmid

    #198581

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BcLF1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBx1-VHH template1-3XMyc-Spacer

    Plasmid

    #176208

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template1-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TAL-L1-1.3-VP64

    Plasmid

    #103033

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3-VP64
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    HA and VP64

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. T7-RelA(S276A)

    Plasmid

    #24153

    Depositor

    Warner Greene

    Article

    Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

    Insert

    RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    S276A

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pCLX-M38s/TOP-dGFP

    Plasmid

    #114295

    Purpose

    Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    dGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    destabilized GFP

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SARS-CoV-2_N_deltaCTD

    Plasmid

    #157871

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domain

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaCTD

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFRT_TO_FlagHA_PRKRA

    Plasmid

    #106111

    Purpose

    Inducible gene expression of FlagHA-PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR4_PRKRA

    Plasmid

    #106110

    Purpose

    Entry vector for PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pALPS tat-P2A-rev

    Plasmid

    #101331

    Purpose

    Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 tat and rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge