We narrowed to 6,654 results for: SIM;

1. pOGG113

   Plasmid

   #133124

   Purpose

   pL0M-PU-NoP, promotorless region used as negative control

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   NoP promotorless region from plasmid pIJJ11268
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_hU6miR-G13-L

   Plasmid

   #25758

   Purpose

   Entry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 13 miR-shRNA
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. MDH1-PGK-FLAG-TAT-SIVmac251

   Plasmid

   #35979

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   SIV strain mac251

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB2801

   Plasmid

   #63656

   Purpose

   pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH104

   Plasmid

   #48262

   Purpose

   Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

   Inserts

   TEF1p 5' fragment
   URA3 5' fragment
   TEF1p 3' fragment
   

   Use

   Routine cloning vector

   Tags

   Expression

   Mutation

   From SK1 background. -343 to -1 relative to TEF1 …

   Promoter

   TEF1p

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2991

   Plasmid

   #63643

   Purpose

   pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQTEV-C9orf58

   Plasmid

   #34819

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   C9orf58 (AIF1L Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TmiR_G13-L_G12-E

   Plasmid

   #25763

   Purpose

   Entry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 13 and 12 miR-shRNA
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. myc-SpELL pBacPAK8

   Plasmid

   #15318

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
   

   Use

   Baculovirus construction

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pCINTBeta1

    Plasmid

    #14067

    Depositor

    Richard Hynes

    Article

    Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

    Insert

    Chicken Beta 1 Integrin (ITGB1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PstI-BglI fragment was blunt end cloned and EcoRI…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pJH124

    Plasmid

    #48259

    Purpose

    Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    Ag TEF1 promoter
    

    Use

    Routine cloning vector

    Tags

    Expression

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    Ag TEF1 promoter

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-SpEAF 60-251 pBacPAK8

    Plasmid

    #15321

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    EAF1 (eaf1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    N terminal deletion lacking first 59 amino acids …

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pHAGE2-EF1alpha TDH

    Plasmid

    #236457

    Purpose

    stable expression of TDH using viral infection

    Depositor

    Michele Pagano

    Article

    Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

    Insert

    TDH (Tdh Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR89B_full_pGCS1

    Plasmid

    #238499

    Purpose

    IVT mRNA of human gene

    Depositor

    Megan Dennis

    Article

    Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

    Insert

    GPR89B (GPR89B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYihI-N-term D-N/E-Q

    Plasmid

    #233095

    Purpose

    Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-term aspartic acids (D) mutated to asparagine (…

    Promoter

    Available since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pYihI-N-term D-A/E-L

    Plasmid

    #233097

    Purpose

    Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-term aspartic acids (D) mutated to alanine (A) …

    Promoter

    Available since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLJC6-NADK2(delta MTS)-3xFLAG

    Plasmid

    #233896

    Purpose

    lentiviral overexpression vector for NADK2 (delta MTS)

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    NADK2 (NADK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    delta MTS (see Depositor Comments)

    Promoter

    Ubc

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR-v1-sgNADK2

    Plasmid

    #233897

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting NADK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    sgRNA targeting NADK2 (NADK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLIB-Strep(II)(N)-PIN1

    Plasmid

    #231724

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    PIN1 (PIN1 Human)
    

    Use

    Tags

    Strep(II)-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA-GTSE1

    Plasmid

    #224719

    Purpose

    For expression of HA-tagged GTSE1 protein

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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