We narrowed to 14,039 results for: TIM

1. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

   Plasmid

   #199248

   Purpose

   Fluorescent labelling of bacterial cells with sfGFP

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized sfGFP gene
   

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJR98

   Plasmid

   #187239

   Purpose

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. A2_uSFFV

   Plasmid

   #153415

   Purpose

   Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   A*02:01-P2A-human beta2 microglobulin (B2M Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Nme2ABE8e

   Plasmid

   #189928

   Purpose

   Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   Nme2ABE8e
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW003-PpsbA-mNeonGreen

   Plasmid

   #205019

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   PpsbA

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

   Plasmid

   #174007

   Purpose

   Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

   Depositor

   Mark Histed

   Article

   LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

   Has Service

   AAV9

   Insert

   jGCaMP8s-P2A-ChrimsonR-ST
   

   Use

   AAV

   Tags

   6xHis and soma targeting motif

   Promoter

   hSyn

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-CjABE8e

   Plasmid

   #189929

   Purpose

   Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   CjABE8e
   

   Expression

   Mammalian

   Mutation

   CjCas9 D8A

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 IST1 (1-366)

   Plasmid

   #193039

   Purpose

   Bacterial expression plasmid for full-length IST1 (1-366).

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid

    #216112

    Purpose

    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LAMP1-RpHLuorin2

    Plasmid

    #171720

    Purpose

    Lysosomal expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. non-targeting control gRNA (BRDN0001487130)

    Plasmid

    #80262

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. non-targeting control gRNA (BRDN0001147380)

    Plasmid

    #80225

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SYN

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    MSCV LTR promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. hPlekhm1-dsRedM

    Plasmid

    #73592

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    dsRed

    Expression

    Mammalian

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pHR-UCOE-SFFV-EGFP

    Plasmid

    #188900

    Purpose

    Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid

    #117699

    Purpose

    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    

    Tags

    HA tag, Myc-NES, and NLS

    Expression

    Mammalian

    Mutation

    codon optimized for murine expression

    Promoter

    None, 2A peptide from porcine teschovirus-1 polyp…

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.4_CEACAM6-His

    Plasmid

    #149337

    Purpose

    for eukaryotic expression and purification of CEACAM6-His6

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Tags

    IL-2 signal peptide and polyhistidine

    Expression

    Mammalian

    Mutation

    point mutation (GGA to GCA) to replace 'G-g…

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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