We narrowed to 5,107 results for: PEP

1. pTAMAHISTEV_PfeAQ482A

   Plasmid

   #128945

   Purpose

   expresses Q482A mutant of PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Use

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75), …

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD47-CD4d3+4-bio

   Plasmid

   #73121

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CD47 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CD47 antigen (CD47 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SENP6 (catalytic domain)

   Plasmid

   #16359

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP6 catalytic domain (SENP6 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 628 to 1112 catalytic domain of human …

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

4. Ntng2.b-AP-His

   Plasmid

   #71995

   Purpose

   Expresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntng2.b (Ntng2 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ntng1.a-AP-His

   Plasmid

   #71984

   Purpose

   Expresses the extracellular region of the Netrin G1, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntng1.a (Ntng1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-HemmarG

   Plasmid

   #31221

   Depositor

   Yuh-Nung Jan

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 May 23. (

   Insert

   CD4-tdGFP (CD4 Aequorea victoria, Fly, Human)
   

   Use

   Tags

   CD4 and EGFP/GFP

   Expression

   Insect

   Mutation

   Fusion of signal peptide of Drosophila Akh gene, …

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. AMA1-bio

   Plasmid

   #47741

   Purpose

   Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised AMA1
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP10-bio

   Plasmid

   #47719

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP10
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUPI hT5-S322-Cyp

   Plasmid

   #80282

   Purpose

   Expresses human TRIM5 CypA fusion

   Depositor

   Jeremy Luban

   Article

   Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

   Insert

   TRIM5 CypA fusion (TRIM5, PPIA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEmerald(luminal)-canxTM

    Plasmid

    #186961

    Purpose

    Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) with ER signal pe…

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. GlycophorinA-CD4d3+4-bio

    Plasmid

    #73093

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinA with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin A (GYPA Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HCMVgO-sfGFP (Merlin)

    Plasmid

    #136452

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+) 6xHis-TEV-IFIT2

    Plasmid

    #53558

    Purpose

    bacterial expression of IFIT2

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT2 (IFIT2 Human)
    

    Use

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTRAP-bio

    Plasmid

    #47746

    Purpose

    Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MTRAP
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lactotransferrin-CD4d3+4-bio

    Plasmid

    #73124

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Lactotransferrin (LTF Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EBA140-bio

    Plasmid

    #47742

    Purpose

    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised EBA140
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pScalps_Puro_mCebpe

    Plasmid

    #79076

    Purpose

    Expression of mouse Cebpe

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp epsilon (Cebpe Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-GFP-EBA175-Flag

    Plasmid

    #154970

    Purpose

    Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    GFP and signal peptide (residues 1 to 32) from TG…

    Expression

    Mammalian

    Mutation

    insert codes for EBA175 residues 1284 to 1462

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEX-CMV-SP-YFP-STM2(15-746) EF hand D80A

    Plasmid

    #18864

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    Stromal interaction molecule 2 (STIM2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    EF hand mutant: D80A. YFP inserted between signal…

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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