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pAAV-Ef1a-Con/Fon oG

Plasmid

#131778

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

rabies glycoprotein - oG

Use

AAV; Intrsect

Tags

none

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

Plasmid

#127312

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98928

Purpose

Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1 and AAV5

Insert

Ruby2-smFP-Flag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Tagged-IRES

Plasmid

#35570

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Insert

Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)

Use

Luciferase

Tags

Firefly Luciferase and MS2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pNvT-MHC::mCh

Plasmid

#67943

Purpose

Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

Depositor

Ulrich Technau

Article

Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

mCherry

Use

Transgenic reporter in the sea anemone, nematoste…

Promoter

Myosin Heavy Chain1 (MyHC1)

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-eGFP-P2A-dTAG-Ing3

Plasmid

#170754

Purpose

Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

eGFP-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

eGFP-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

none

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ErbB3

Plasmid

#197362

Purpose

Encoding human ErbB3.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB3 (ERBB3 Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMCP-Luc (MCP-1 promoter in pGL3-basic)

Plasmid

#40324

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pV1393

Plasmid

#111430

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_erbb2_cmlc2-nmKate

Plasmid

#238409

Purpose

Drives expression of 3 different gRNAs targeting erbb2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-SF3B1-K700E-reporter

Plasmid

#200318

Purpose

A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

Depositor

Lili Wang

Article

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Insert

BSD-2A-EGFPi

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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145-pGL3ctrl-3' UTR

Plasmid

#28175

Depositor

Michael Kastan

Article

Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

Insert

Luciferase flanked by tp53 UTRs (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Note that insert length is based on sum of the 2 …

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMZ828

Plasmid

#155322

Purpose

Constitutive expression of PhyB(1-650)-VP16 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

Plasmid

#176704

Purpose

neural activity dependent release of sbGLuc from presynaptic vesicles

Depositor

Ute Hochgeschwender

Article

Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

Inserts

Gaussia luciferase, slow burn
dTomato

Use

AAV

Tags

P2A and hPOMC1-26

Promoter

hSyn

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

Plasmid

#127332

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTETRIS-cargo

Plasmid

#126033

Purpose

Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Type

Empty backbone

Use

Luciferase; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.11-Cd36-promoter

Plasmid

#138572

Purpose

Firefly luciferase reporter fused with a 1296 bp fragment of mouse Cd36 promoter

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

SV40

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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H52-pUCM-hNGN2-inv

Plasmid

#110492

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

Depositor

Michael Ward

Inserts

Neurogenin 2 (NEUROG2 Human)
mcherry
rtTA3G

Use

CRISPR, Cre/Lox, and TALEN

Expression

Mammalian

Promoter

CAG, EF-1 alpha, and TRE3G

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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