165,075 results

1. pAAVS1-P-MCS

   Plasmid

   #80488

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Expression

   Mammalian

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET Nucleolin

   Plasmid

   #13037

   Depositor

   Peter Newburger

   Article

   Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

   Insert

   Nucleolin (NCL Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

   Plasmid

   #203908

   Purpose

   Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   Gal4UAS_dGFP_PGK_iRFP
   

   Expression

   Mammalian

   Promoter

   5xUAS and PGK

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-eGFP

   Plasmid

   #36083

   Purpose

   3rd generation lentiviral transfer plasmid

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Hygro-mTagBFP2

   Plasmid

   #99374

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-IP-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #84572

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2029

   Plasmid

   #143566

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMARCA5 (SMARCA5 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Expression

   Mammalian

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRUPATH Triple Ga11

   Plasmid

   #196059

   Purpose

   Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha11-RLuc8 (GNA11 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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