We narrowed to 11,414 results for: Vars;
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Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/F-tractin=mStayGold
Plasmid#212019PurposeFor filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertF-tractin
TagsmStayGoldExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-7xHis-TEV-hexa
Plasmid#222868PurposeExpresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
Tags7xHis and MBPExpressionBacterialMutationC19V, L56V, C110V, C130S, S135G, S219V. Insert in…Available SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP
Plasmid#169327PurposeExpression of CEP41-HaloTag7 in mammalian cellsDepositorAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorInsert6xCRE-CMVmin-luc2
ExpressionMammalianPromoterCMV minimalAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23b-2E2-HA-mEGFP-6xHis
Plasmid#129594PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRIP-CMV-GFP-FLAG-cGAS
Plasmid#86675PurposeExpresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
TagsmStayGold(c4)ExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TadA8e-PcrA M6
Plasmid#221229PurposeExpress HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.DepositorInsertTadA8e-PcrA M6
ExpressionMammalianAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2 ER:ras
Plasmid#67844Purpose4-hydroxytamoxifen inducible ER:ras fusion proteinDepositorInsertER1a H-RasG12V fusion
ExpressionMammalianMutationG525R renders the HBD largely insensitive to 17ß‐…PromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
WT GBA pcDNA3.1
Plasmid#188580PurposeEctopic expression of wild-type GBADepositorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorAvailable SinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-SpCas9(D10A)-T2A-EGFP
Plasmid#221230PurposeExpress nCas9 nickase (D10) with GFP reporterDepositorInsertSpCas9(D10A)-T2A-EGFP
ExpressionMammalianMutationD10AAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PEmax-2A-MLH1dn-2A-BSD
Plasmid#234072PurposeFor lentiviral expression of PEmax with MLH1dn and blasticidin resistanceDepositorInsertPEmax-2A-MLH1dn
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF-1α core promoterAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)
Plasmid#188375PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUbC-OsTIR1-myc-IRES-scFv-sfGFP
Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)Available SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mCh
Plasmid#129596PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
ExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP
Plasmid#169328PurposeExpression of HaloTag9 located at the microtubules of mammalian cellsDepositorInsertCEP41-HaloTag9 (CEP41 Human)
TagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only