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pBad-UXPS

Plasmid

#172138

Purpose

bacteria expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Publication

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Expression

Bacterial

Promoter

araBAD

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Expression

Mammalian

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Publication

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUASTattb-cAMPr Y205A, R626A

Plasmid

#84932

Purpose

cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

Depositor

Justin Blau

Publication

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr Y205A R626A

Expression

Insect

Mutation

PKA-C Y205A, PKA-R R626A

Promoter

5X UAS

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_508-SV40-GFP

Plasmid

#75138

Purpose

Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_508

Use

Retroviral

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGGE001

Plasmid

#48839

Purpose

Provides the rbcS terminator as GreenGate module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

rbcS terminator

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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placZα-tdMCP

Plasmid

#191132

Purpose

Expression of tdMCP from a lacZα inducible promoter

Depositor

Ariel Lindner

Publication

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

dMCP_1

Use

Synthetic Biology

Promoter

lac-inducible promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hGFAP-Pink Flamindo-WPRE-SV40

Plasmid

#178790

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Publication

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

Pink Flamindo

Use

AAV

Promoter

hGFAP

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe

Plasmid

#170623

Purpose

For the mammalian expression of mLOV2GIVe

Depositor

Mikel Garcia-Marcos

Publication

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCFJ2359 - intron GG2 - 250bp

Plasmid

#159878

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG2 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPI226

Plasmid

#113228

Purpose

safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct

Use

Safe haven knock-in plasmid for the act5 locus, l…

Tags

mCherry

Promoter

act5

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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CCR5_gRNA_pX459

Plasmid

#192268

Purpose

gRNA expression for CRISPR Genome Editing of CCR5

Depositor

Shantanu Chowdhury

Publication

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

CCR5 guide RNA

Use

CRISPR

Expression

Mammalian

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBad-UGAcS

Plasmid

#172137

Purpose

bacteria expression plasmid for a UDP-GlcNAc sensor

Depositor

Huiwang Ai

Publication

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UGAcS

Expression

Bacterial

Mutation

H605Q- please see depositor comments

Promoter

araBAD

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-VRER

Plasmid

#159984

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Publication

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-VRER

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_AtPIF6_X

Plasmid

#131182

Purpose

Expresses AtPIF6 (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPIF6

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_2

Plasmid

#101077

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Expression

Mammalian

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLAC

Plasmid

#45072

Purpose

lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

Depositor

Gordon Laurie

Publication

Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

Insert

Lacritin (LACRT Human)

Use

Protein expression vector

Tags

Intein

Expression

Bacterial

Promoter

T7

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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