We narrowed to 10,106 results for: Uty

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p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEe

Plasmid

#193836

Purpose

Express TadCBEe (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDe-SpCas9D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

Plasmid

#205320

Purpose

All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISAba30 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2b_M18

Plasmid

#105931

Purpose

Expression plasmid coding for heavy chain of mouse IgG2b antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b (Ighg2b Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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RNF130 shRNA-TRE

Plasmid

#225336

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 shRNA (Rnf130 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR-mCherry

Plasmid

#204868

Purpose

Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

M. mazei pyrrolysyl tRNA

Use

AAV

Expression

Mammalian

Mutation

U25C

Promoter

U6

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-EcYtR-mCherry

Plasmid

#217362

Purpose

Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAG225 HIPK1 3'UTR mut

Plasmid

#12044

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

HIPK1 3'UTR mut (HIPK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
hP2X3-cALFA

Plasmid

#160499

Purpose

For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

human P2X3 (P2RX3 Synthetic, Human)

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

ΔN5ΔC33-T13P/S15V/V16I

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAG177 HIPK1 3'UTR mut

Plasmid

#12035

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N10 3'UTR mut (HIPK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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SPLINTR mCHERRY v1 backbone

Plasmid

#179778

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCW17 - pAdapter

Plasmid

#174664

Purpose

Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.

Depositor

Katherine Berry

Article

Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

Insert

MS2 coat protein

Tags

λCI

Expression

Bacterial

Mutation

V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

Promoter

Synthetic Constitutive Promoter

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET23a-His-hRanGAP1tail

Plasmid

#53139

Purpose

E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanGAP1 (RANGAP1 Human)

Tags

His

Expression

Bacterial

Mutation

C-terminal fragment (amino acids 398-587)

Promoter

T7

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pCVL.Active/Repressed TLR (Sce)

Plasmid

#45575

Purpose

Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

eGFP with I-Sce I TS
+3 mCherry

Use

Lentiviral

Tags

T2A dislinker and iRFP

Expression

Bacterial and Mammalian

Mutation

embedded I-Sce I TS from 163-185

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Cdc42-Q61L

Plasmid

#214288

Purpose

Express constitutively active Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active Cdc42 (CDC42 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-Q61L

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAG70 BDNF 3'UTR

Plasmid

#12021

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

BDNF 3'UTR (BDNF Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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L309-myc-seipin-WT

Plasmid

#64061

Purpose

myc-tagged murine seipin (wild type); lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

Insert

bscl2 (Bscl2 Mouse)

Use

Lentiviral

Tags

myc epitope

Expression

Mammalian

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pET21-GFP-AURKA

Plasmid

#99875

Purpose

Expression of GFP-AURKA for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Bacterial

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

Plasmid

#166421

Purpose

Express pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Naegleria fowleri PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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