We narrowed to 9,933 results for: Uty

1. pET28b(+)-SPN+IFS

   Plasmid

   #83372

   Purpose

   Expresses SPN and IFS complex in E. coli cell

   Depositor

   SeWon Suh

   Article

   Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

   Inserts

   SPN
   IFS
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BICD2N

   Plasmid

   #64070

   Purpose

   N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

   Depositor

   Andrew Carter

   Article

   Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

   Insert

   BICD2N (1-400) (Bicd2 Mouse)
   

   Use

   Tags

   GFP and His-ZZ-TCS

   Expression

   Insect

   Mutation

   Codon optimized for Sf9 expression

   Promoter

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA0055

   Plasmid

   #131774

   Purpose

   Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer CASS5a guide (gCASS5a) and 5' sgRNA
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAGGS-mNAGA

   Plasmid

   #196448

   Purpose

   Expresses modified α-N-acetylgalactosaminidase in mammalian cells

   Depositor

   Yuichiro Miyaoka

   Article

   Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

   Insert

   modified alpha-N-acetylgalactosaminidase (NAGA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T1-Claspin-MΔN

   Plasmid

   #31624

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

   Insert

   Claspin (CLSPN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 899-1206 only

   Promoter

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pMP71-mtGFP-llo-lama4-alg8

   Plasmid

   #174605

   Purpose

   expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain

   Plasmid

   #183397

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of human caspase-4 fused to catalytic domain of canine caspase-1/4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac HT JS-Munc18b

   Plasmid

   #135554

   Purpose

   Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18b (Stxbp2 Rat)
   

   Use

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

   Plasmid

   #105025

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfcd (Th1l Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

    Plasmid

    #105026

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGP67A-hBAI3-HormR-GAIN

    Plasmid

    #37848

    Depositor

    Axel Brunger

    Article

    Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

    Insert

    BAI3 (BAI3 Human)
    

    Use

    Baculovirus transfer vectors

    Tags

    8xHIS

    Expression

    Mutation

    HormR and GAIN domains only (aa498–868

    Promoter

    Polyhedrin

    Available Since

    July 6, 2012

    Availability

    Academic Institutions and Nonprofits only

12. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Use

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. CMV epArcLight

    Plasmid

    #85803

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    epArcLight
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; ecliptic pHluorin…

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMX-eSOX17WHC

    Plasmid

    #118839

    Purpose

    Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX17WHC (Sox17 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    WHC: L46W, V53H, E57C

    Promoter

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKI-cjFOXL2-coemiRFP670-PGK-Puro

    Plasmid

    #186168

    Purpose

    Knock-in gene targeting in marmoset cells at FOXL2 locus

    Depositor

    Sho Yoshimatsu

    Article

    Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

    Insert

    emiRFP670
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pg-dsRedXP-HIF-2alpha

    Plasmid

    #87209

    Purpose

    N-terminally dsRedXP-tagged human HIF-2a/EPAS

    Depositor

    Violaine Sée

    Article

    Taylor et al Open Biol. 2016 Sep;6(9). pii: 160195.

    Insert

    HIF-2a (EPAS1 Human)
    

    Use

    Tags

    dsRedXP

    Expression

    Mammalian

    Mutation

    Q868E

    Promoter

    CMV

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Zebrafish PAC (fPAC)

    Plasmid

    #129498

    Purpose

    Expresses zebrafish TMEM206. In pcDNA3.1(+) backbone

    Depositor

    Zhaozhu Qiu

    Article

    Yang et al Science. 2019 Apr 26;364(6438):395-399.

    Insert

    zebrafish PAC (pacc1 Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-kcnj11

    Plasmid

    #173139

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj11 (kcnj11 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. cARA12-mRFP

    Plasmid

    #181961

    Purpose

    ARA12 protein tagged with mRFP, under control of TBA2 promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    ARA12 (ARA12 Mustard Weed)
    

    Use

    Tags

    mRFP

    Expression

    Plant

    Mutation

    Promoter

    TBA2

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. mOC-STAMP_3xFlag-Myc-CMV26

    Plasmid

    #73577

    Purpose

    Express mouse OC-STAMP in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Insert

    OC-STAMP (Ocstamp Mouse)
    

    Use

    Tags

    3x Flag and c-Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only