19,640 results

1. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

   Plasmid

   #125713

   Purpose

   Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV1 and AAV5

   Insert

   eOPN3
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   hSyn1

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC1-HyPer-Red-C199S

   Plasmid

   #48252

   Purpose

   Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HYPER-RED-C199S
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-FLEX-GTB

   Plasmid

   #26197

   Depositor

   Edward Callaway

   Article

   Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

   Insert

   Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Mutation

   TVA, rabies glycoprotein are mammalian codon opti…

   Available Since

   Nov. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. FU-H2B-GFP-IRES-Puro

   Plasmid

   #69550

   Purpose

   Lentiviral, selectable fluorescent GFP tagging of nuclei.

   Depositor

   Charles Gersbach

   Insert

   Histone 2B - green fluorescent protein fusion
   

   Use

   Lentiviral

   Tags

   GFP fusion

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGD-GmR

   Plasmid

   #74106

   Purpose

   Plasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites

   Depositor

   Hans-Martin Fischer

   Article

   Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

   Insert

   aacC1
   

   Use

   Bacterial vector based on pmb1 replicon

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-hChR2-H134R-tdTomato

   Plasmid

   #28017

   Purpose

   AAV-mediated expression of hChR2(H134R) for neuronal excitation.

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Has Service

   AAV Retrograde

   Insert

   humanized ChR2-H134R-tdTomato
   

   Use

   AAV; Adeno associate viral

   Tags

   tdTomato

   Mutation

   H134R

   Promoter

   CAG

   Available Since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CD14

   Plasmid

   #13645

   Depositor

   Doug Golenbock

   Insert

   CD14 (CD14 Human)
   

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4 HisMax-V5-GFP-RRBP1

   Plasmid

   #92150

   Purpose

   Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   RRBP1 (RRBP1 Human)
   

   Tags

   Calreticulin signal peptide, EGFP (A207K mutation…

   Expression

   Mammalian

   Mutation

   In RRBP1: N951S and T1329L. E1279 is also missing.

   Promoter

   CMV

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Tsg101

    Plasmid

    #21505

    Depositor

    James Hurley

    Article

    Lee et al Science. 2008 Oct 24. 322(5901):576-80.

    Insert

    Tsg101 (TSG101 Human)
    

    Tags

    Cherry*

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191796

    Purpose

    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13X
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    R84A, H89A, R739A, R740A, H745A, H746A, H747A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRG2

    Plasmid

    #104174

    Purpose

    sgRNA expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_SFFv_4D5-WT-Highest-CAR_RHL004

    Plasmid

    #164826

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAY5 (mAID-EGFP-NLS piggyBac)

    Plasmid

    #140532

    Purpose

    mAID-EGFP-NLS piggyBac

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEmerald-Calreticulin-N-16

    Plasmid

    #54023

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mucolipin1-pHcRed C1

    Plasmid

    #62959

    Purpose

    full length human Mucolipin-1 cloned into pHcRed1 C1

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Tags

    HcRed1

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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