20,049 results

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pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pRL623

Plasmid

#58494

Purpose

helper plasmid for bacterial conjugal DNA transfer

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Insert

encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120

Available Since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Rtn4a

Plasmid

#86683

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 deltaN89 Beta-catenin

Plasmid

#19288

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal truncation, deleted aa 1-89

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-B mCherry

Plasmid

#108857

Purpose

Encodes for the fluorescent protein mCherry

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

mCherry

Expression

Bacterial

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFBR2

Plasmid

#116800

Purpose

Lentiviral expression of TGFBR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM33

Plasmid

#65398

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM33 (TRIM33 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

Plasmid

#205555

Purpose

Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP

Plasmid

#160697

Purpose

Expresses GFP in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.hSyn.Cre.WPRE.hGH

Plasmid

#105553

Purpose

AAV expression of Cre from hSyn promoter

Depositor

James M. Wilson

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo4

Plasmid

#17549

Depositor

Domenico Accili

Insert

FoxO4 (FOXO4 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pBS-hBAF60b

Plasmid

#21035

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60b (SMARCD2 Human)

Use

Phagemid

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pCR2.1 Clover-LMNA Donor

Plasmid

#122508

Purpose

Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)

Use

Homology repair template plasmid (donor plasmid)

Tags

Clover

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG16_2_synNotch_Gal4VP64

Plasmid

#85421

Purpose

Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

LaG16_2 GFP nanobody synNotch Gal4VP64

Use

Lentiviral

Promoter

PGK

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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hSERT pcDNA3

Plasmid

#15483

Depositor

Randy Blakely

Article

Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

Insert

hSERT (SLC6A4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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Cavb3

Plasmid

#26574

Depositor

Diane Lipscombe

Insert

calcium channel beta subunit-III (Cacnb3 Rat)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pTetOne-mCherry-cNLS

Plasmid

#138527

Purpose

Inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE3Gs

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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