We narrowed to 83,138 results for: ELL

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pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64

Plasmid

#85423

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-5 synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLifeAct-mScarlet3_N1

Plasmid

#189767

Purpose

Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LifeAct-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSens-4OmeNB

Plasmid

#216231

Purpose

Expresses GFP in response to 4'-O-Methylnorbelladine

Depositor

Simon d'Oelsnitz, Andrew Ellington

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Inserts

RamR-4NB2
sfGFP

Use

Synthetic Biology

Mutation

K64T, L67M, C135D, S138G

Promoter

J23103 and Pramr

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64

Plasmid

#85425

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-8_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only
tph-1(short)::rpl-22-HA

Plasmid

#122203

Purpose

C. elegans tagged ribosome expression plasmid

Depositor

Steven Flavell

Article

Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

Insert

tph-1(short)::rpl-22-HA

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-d2GFP-ZEB1 3'UTR

Plasmid

#79601

Purpose

FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP-Zeb1 3'UTR

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-LaminB_C1

Plasmid

#189776

Purpose

Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-LaminB

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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SOPP3-HA-nes

Plasmid

#229071

Purpose

Expresses SOPP3 in the cytosol of mammalian cells

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express SOPP3 in the cytosol of mammalian cells

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

Plasmid

#85424

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-7_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGuide

Plasmid

#64711

Purpose

Cloning and expression of Streptococcus pyogenes guide RNA

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Streptococcus pyogenes guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-alphaTubulin_C1

Plasmid

#189768

Purpose

Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-alphaTubulin

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLOX-CW-CRE

Plasmid

#12238

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry

Plasmid

#85430

Purpose

[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-10 T2A PD-L1

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinD-IRES-Puro

Plasmid

#213114

Purpose

Expression of untagged MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTK-Twist

Plasmid

#36977

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Twist (Twist1 )

Use

Lentiviral

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only
pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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