We narrowed to 30,686 results for: PLE;

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FKBP-EGFP-RanGAP

Plasmid

#175236

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-V9

Plasmid

#173797

Purpose

pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation

Depositor

Daniel Sloan

Article

Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

Inserts

aadA
ClpP1

Expression

Bacterial and Plant

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-V7

Plasmid

#173796

Purpose

pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene latifolia clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation

Depositor

Daniel Sloan

Article

Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

Inserts

aadA
ClpP1

Expression

Bacterial and Plant

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-V6

Plasmid

#173795

Purpose

pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene conica clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation

Depositor

Daniel Sloan

Article

Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

Inserts

aadA
ClpP1

Expression

Bacterial and Plant

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-V4

Plasmid

#173794

Purpose

pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Nicotiana tabacum clpP1 gene with native regulatory elements and flanking regions for tobacco plastome transformation

Depositor

Daniel Sloan

Article

Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

Inserts

aadA
ClpP1

Expression

Bacterial and Plant

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pG418R-MCS-LexA-VP16-MiniWhite

Plasmid

#165894

Purpose

G418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2267

Plasmid

#158392

Purpose

High Copy number plasmid containing Ple341 (PCP2 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

Unspecified

Promoter

Ple341

Available Since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2279

Plasmid

#158399

Purpose

High Copy number plasmid containing Ple349 (PDE6H MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple349 (PDE6H Human)

Use

Unspecified

Promoter

Ple349

Available Since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pKD284

Plasmid

#125073

Purpose

Expression of SO_4488 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4488 (SO_4488 )
lacI (lacI S286L- Please see depositor comments below)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKD278

Plasmid

#125070

Purpose

Expression of SO_2192 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_2192 (SO_2192 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

Mutation in LacI S286L- Please see depositor comm…

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR42

Plasmid

#106022

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR42; Opto-GPR42; B12)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR42 (GPR42 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-biotin-myc- mCE

Plasmid

#82475

Purpose

Expresses mRNA capping enzyme containing N terminal biotin and myc tag

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

Biotin (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMflCT-o4

Plasmid

#101313

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0-GL-2-3 perfect copies

Plasmid

#82441

Purpose

Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Mutation

The complementary region for GL2 siRNA control wa…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pVH003

Plasmid

#80397

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold - Venus YFP
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

Fused by GS linker to LZx domain and Venus has N-…

Expression

Bacterial

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSR11

Plasmid

#69154

Purpose

read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
fzr-1 (fzr-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

fzr-1 and rps-27

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDD2000

Plasmid

#101197

Purpose

Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]

Use

Luciferase

Availability

Academic Institutions and Nonprofits only

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pML107

Plasmid

#67639

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52 and pTDH3 (aka GAP promoter)

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞IRFP

Plasmid

#154017

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

IRFP670

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pML104

Plasmid

#67638

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52 and pTDH3

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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