We narrowed to 6,088 results for: cat.2

1. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

   Plasmid

   #190153

   Purpose

   Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only AA #1-94

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSI2-DCD

   Plasmid

   #166968

   Purpose

   Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

   Depositor

   Michael Kharas

   Article

   Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

   Insert

   MSI2-DCD
   

   Use

   Retroviral

   Tags

   MSI2-DCD

   Expression

   Mammalian

   Mutation

   N495S

   Promoter

   MSCV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUPER-retro-puro-shOrai1

   Plasmid

   #89933

   Purpose

   Knockdown human Orai1 expression

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   Orai1 shRNA (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-iCAP-PHP.B3

   Plasmid

   #103004

   Purpose

   non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B3 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-iCAP-PHP.B2

   Plasmid

   #103003

   Purpose

   non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B2 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T Doc2beta C2AB(125) G361C

   Plasmid

   #134694

   Purpose

   Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Double C2 protein beta (Doc2b Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G361C, C145A, C217A, C249A, C290A, C338A, C387A

   Promoter

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_CASP2_Peptidase-C14

   Plasmid

   #109996

   Purpose

   Protein expression and purification of CASP2_Peptidase-C14

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   CASP2_Peptidase-C14 (CASP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

   Plasmid

   #212738

   Purpose

   Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-NLS-VSV and myc

   Expression

   Yeast

   Mutation

   HOIP(C885A)

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YW347_Ptbb2UTR-loxP-RFP-miniMOS

   Plasmid

   #196064

   Purpose

   a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

   Depositor

   Wenxing Yang

   Article

   Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

   Inserts

   myo2p driven tagRFP
   NeoR
   Minimal Mos1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   myo-2

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCB’ CsoS2 ΔNTD

    Plasmid

    #162705

    Purpose

    Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deletion of residues 2-256 of CsoS2, Y131D

    Promoter

    PLtet0-1 promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T Doc2beta C2AB(125) H158C

    Plasmid

    #134691

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H158C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T Doc2beta C2AB(125) F222C

    Plasmid

    #134692

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F222C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-4T Doc2beta C2AB(125) A298C

    Plasmid

    #134693

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A298C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PFKFB2 gRNA (BRDN0001146926)

    Plasmid

    #77686

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PFKFB2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PFKFB2 (PFKFB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HLA-cMyc-EcopT1R1

    Plasmid

    #113962

    Purpose

    mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R1 (TAS1R1 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    truncate N-terminal 24 residues

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CCR2-Tango

    Plasmid

    #66239

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR2 (CCR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDiCre-mCherry

    Plasmid

    #164572

    Purpose

    Integration of a DiCre cassette in PbGFP parasite genome

    Depositor

    Olivier Silvie

    Article

    Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

    Inserts

    FKBP12-CRE59
    FRB-CRE60
    mCherry
    Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Bidirectional PbEF1alpha promoter (PBANKA_1133300…

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GPR37L1-Tango

    Plasmid

    #66356

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR37L1 (GPR37L1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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