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Plasmid#105524PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLUT7 HA-GLI1
Plasmid#62970PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-PXN
Plasmid#227316PurposeDonor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)DepositorInsertPXN Homology Arms flanking a mStayGold Tag (PXN Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psigma1-EGFP
Plasmid#53611PurposeExpresses sigma1 of adaptor protein of AP1DepositorInsertsigma 1 (Ap1s1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pR26-CMVconst
Plasmid#127373PurposeAllows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMutationPromoterCMV enhancer/promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
BirA-Myc_N_MYC
Plasmid#167715PurposeExpresses MYC in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsMyc-BirAExpressionMammalianMutationno mutationsPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
UseTagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-dTomato-2xrGBD
Plasmid#222638PurposeRho reporterDepositorInsertmiRFP680-NES-p2A-dTomato-2xrGBD
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcg 2.0-BFP-circ-arRNA backbone
Plasmid#213965Purposepcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences oDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABEPuro-HA-GLI1
Plasmid#62967PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_IRF8
Plasmid#167763PurposeExpresses IRF8 in mammalian cellsDepositorInsertIRF8 (IRF8 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-CAHS3-mEGFP
Plasmid#205006PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.DepositorInsertRvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_mNeonGreen_FLAG_C
Plasmid#218073PurposeRESOLUTE cloning plasmidDepositorTypeEmpty backboneUseLentiviralTagsFLAG-mNeonGreen-V5ExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
M76 pIRESpuro-GLUE-hDsh2
Plasmid#15101DepositorInsertDsh2 (DVL2 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 barr1 HA
Plasmid#14693DepositorInsertbeta arrestin 1 (Arrb1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
aav-tnt-dnmrtfa-gfp
Plasmid#165035PurposeAAV-based delivery of dnMRTFA-GFP into cardiomyocytes in vivoDepositorInsertdnMRTFA
UseAAVTagsGFPExpressionMutationdeletion of 100 N-terminal amino acidsPromotercTNTAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SIRT2-Flag
Plasmid#102624PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorInsertSIRT2 (SIRT2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM4787
Plasmid#120088PurposeCyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).DepositorInsertPlasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only