We narrowed to 9,400 results for: Coli

1. pGEX-4T-3-SH3BP1

   Plasmid

   #136647

   Purpose

   Expression of SHBP1-GST fusion protein in E. coli

   Depositor

   Karl Matter, Maria Balda

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1 (SH3BP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSP2

   Plasmid

   #26282

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP2
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of dimer of deletion mutant (1-43)…

   Promoter

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-B4E

   Plasmid

   #162067

   Purpose

   For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

   Depositor

   Karen Polizzi

   Article

   Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1

   Insert

   ampR-eIF4E fusion protein
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   eIF4E incorporating the K119A mutation, which is …

   Promoter

   T7

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC-crRNA-Km

   Plasmid

   #158712

   Purpose

   Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   AcGFP (for crRNA cloning)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ARH2-c003

   Plasmid

   #173094

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRHL1 (ADPRHL1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E2-K354, codon optimized

   Promoter

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-ZETAipi

   Plasmid

   #53284

   Purpose

   Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   pds
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MDO2-c004

   Plasmid

   #173104

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K7-D243

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEAST_J23101-amidase

   Plasmid

   #128135

   Purpose

   The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   amidase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28a-His6-SUMO-VAMP3

   Plasmid

   #135552

   Purpose

   Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP3
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRCHisB-TRF2∆M

    Plasmid

    #50489

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Myb (TERF2 Human)
    

    Use

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-437

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAtCHYb1Δ129N

    Plasmid

    #53367

    Purpose

    Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    chyB1 (BETA-OHASE 1 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted codons for the first 129 amino acids

    Promoter

    Trc

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGG-GFP-PM-TurboID

    Plasmid

    #209402

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    cTP-3XHA-TurboID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGG-PM-TurboID

    Plasmid

    #209395

    Purpose

    Transiently expressing TurboID fused with a plasma menbrane targeting peptide in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid

    #73223

    Purpose

    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    dCas9 from S. pyogenes
    

    Use

    Tags

    c-myc

    Expression

    Bacterial

    Mutation

    Silent mutation at bp 1341 A->C to remove an E…

    Promoter

    PL22

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-C4-mCherry

    Plasmid

    #66530

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter C4

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - C4

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pANTO5

    Plasmid

    #131106

    Purpose

    To generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integrase

    Depositor

    Riccardo Manganelli

    Article

    Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

    Insert

    tetR gene by pFRA61 (Boldrin et al 2010)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTorPE-IP2.0

    Plasmid

    #109217

    Purpose

    Express IP2.0 in E. coli

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pThuS

    Plasmid

    #171674

    Purpose

    ThuS expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase ThuS
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMVS-V1

    Plasmid

    #183094

    Purpose

    Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

    Depositor

    Lars Angenent, Bastian Molitor

    Article

    Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

    Inserts

    pME2001
    thermostable neomycin phosphotransferase gene
    

    Use

    Bacterial propagation and conjugative

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. hisSUMO-ZFP57

    Plasmid

    #102716

    Purpose

    Express DNA binding domain of human Zinc finger protein 57 in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    Zinc finger protein 57 (ZNF57 Human)
    

    Use

    Tags

    hisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. GatA_pET29b

    Plasmid

    #215406

    Purpose

    Expression construct for GatA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    GatA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMGA-plux-sfGFP

    Plasmid

    #139936

    Purpose

    AHL-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

    Depositor

    Christopher Voigt

    Article

    Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

    Insert

    LuxR-Plux-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

    Plasmid

    #139788

    Purpose

    Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHis8-4b-SaDAH

    Plasmid

    #140128

    Purpose

    Expresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase from Sinomenium acutum
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. Gi-c009

    Plasmid

    #173079

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    full length, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-28a+GFP-CAMSAP2 CKK

    Plasmid

    #59032

    Purpose

    expresses CKK domain of CAMSAP2 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CKK domain (CAMSAP2 Human)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pGG-NES-TurboID

    Plasmid

    #209393

    Purpose

    Transiently expressing TurboID fused with a nuclear export signal (NES) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    NES-3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAC-PHYT

    Plasmid

    #53300

    Purpose

    Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pENV8(GAAA)-GFPuv

    Plasmid

    #70128

    Purpose

    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-28a-GFP-Patronin CC 868–1457

    Plasmid

    #59053

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only