We narrowed to 24,518 results for: Spr;

1. TNIK gRNA (BRDN0001146045)

   Plasmid

   #75849

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TNIK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TNIK (TNIK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. UAS-FRT-GFP-FRT-u(M)Cas9

   Plasmid

   #127388

   Purpose

   Cas9 inducible by removal of a GFP flip-out cassette

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   FRT-GFP-FRT-uORF(M)Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB1163

   Plasmid

   #210032

   Purpose

   Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas11-P2A-Cas6-T2A-Cas3
   rtta-T2A-blast
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHD-SPARC2-S-LexA::p65

   Plasmid

   #133564

   Purpose

   Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   20X UAS

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-nEF-dCas9

   Plasmid

   #106430

   Purpose

   Expresses SpdCas9 from the nEF promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   nEF-dCas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Promoter

   nEF

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Puro_siKO

   Plasmid

   #86696

   Purpose

   Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   H1 TO

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCKO_Luciferase_sgRNA

   Plasmid

   #74190

   Purpose

   lentiviral vector expressing sgRNA targeting Luciferase

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Luciferase sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_Anc689-NG-nCas9

   Plasmid

   #163527

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-itis pBE-t

   Plasmid

   #195342

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA ctctacgcgggtcttgtagt
   

   Use

   CRISPR

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP1101

    Plasmid

    #65773

    Purpose

    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, and T1337R mutations in C…

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM-Cas9HF1-indRecA56

    Plasmid

    #102294

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-itis pBE-nt

    Plasmid

    #195343

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    

    Use

    CRISPR

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAaePUb::Cas9-2A-Neo

    Plasmid

    #176680

    Purpose

    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAH-CTX1-rhadCas9

    Plasmid

    #129391

    Purpose

    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Burkholderia cenocepacia codon-optimized dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Entire dCas9 codon optimized for the GC-rich B. c…

    Promoter

    PrhaBAD

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_KMT2B_2

    Plasmid

    #101075

    Purpose

    Encodes gRNA for 3' target of human KMT2B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KMT2B (KMT2B Human)
    

    Use

    CRISPR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458_KMT2B_1

    Plasmid

    #101074

    Purpose

    Encodes gRNA for 3' target of human KMT2B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KMT2B (KMT2B Human)
    

    Use

    CRISPR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHD-SPARC2-D-LexA::p65

    Plasmid

    #133562

    Purpose

    Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHD-SPARC2-I-LexA::p65

    Plasmid

    #133563

    Purpose

    Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV_APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163533

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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