We narrowed to 13,291 results for: cDNA

1. pcDNA5-FRT/T0-Flag-53BP1

   Plasmid

   #52507

   Purpose

   mammalian expression vector of 53BP1

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V128A and siRNA resistant sequence: ACGCGCTGACGAC…

   Promoter

   CMV/TO

   Available Since

   March 26, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1-Y1175F-VEGFR2-mCherry

   Plasmid

   #139608

   Purpose

   Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6 MCV LTco S220A

   Plasmid

   #112193

   Purpose

   Expression of MCPyV LT mutant (Ser 220 to Ala)

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCPyV LT codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/HA-FLAG RBP2 H483A

   Plasmid

   #14801

   Depositor

   William Kaelin

   Article

   Klose et al Cell. 2007 Mar 9. 128(5):889-900.

   Insert

   Retinoblastoma Binding Protein 2 (KDM5A Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Changed His 483 to Ala, R46W

   Promoter

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-nAChr-Alpha6-GFP

   Plasmid

   #50486

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   GFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the GFP open…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-PHLPP1 N-terminal truncation

   Plasmid

   #22404

   Purpose

   Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

   Depositor

   Alexandra Newton

   Article

   Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

   Insert

   PHLPP1 (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1-nAChr-beta3-YFP

   Plasmid

   #50484

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   YFP fusion in M3-M4 loop (after residue P379). O…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5/FRT EGFP-miniAID

   Plasmid

   #101714

   Purpose

   For amplification of a EGFP-miniAID for N-terminal tagging

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   EGFP-miniAID
   

   Use

   Tags

   EGFP-miniAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid

    #136451

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid

    #50480

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    mCherry fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+)/AUG-nLuc-3XFLAG

    Plasmid

    #127299

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LARG mCherry sspB in pcDNA3.1

    Plasmid

    #90459

    Purpose

    RhoA mediated cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Inserts

    LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
    mCherry
    sspB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R73Q

    Promoter

    CMV

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-N-mEGFP-PARP7

    Plasmid

    #178004

    Purpose

    Expresses N-terminally mEGFP-tagged PARP7

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP7 (TIPARP Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid

    #136447

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-716). Codon-optimize…

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Flag TSC2 (S939A)

    Plasmid

    #14132

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A.

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(-) MID49 4xMyc Hisx6

    Plasmid

    #44596

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD49 (Mief2 Mouse)
    

    Use

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-N-Myc_1-560 USP7

    Plasmid

    #131244

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 1 - 560. This truncati…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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