We narrowed to 29,518 results for: REP

1. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

   Plasmid

   #50030

   Purpose

   Expression of cytosolic S. pyogenes SrtA in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   SrtA(strep)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-DEST-Strep2

   Plasmid

   #119753

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Use

   Tags

   GST and Strep-tag II fusion tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA4.TO-ORF57-2xCSTREP

   Plasmid

   #136217

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF57
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP L138 Reporter

   Plasmid

   #119130

   Purpose

   Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   mCherry and eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   eGFP L138S

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/TO-UBXD7-Strep-HA

   Plasmid

   #113479

   Purpose

   Expression of human UBXD7 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD7 (UBXN7 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   A834C silent mutation, wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dead Streptavidin-SpyTag

   Plasmid

   #59548

   Purpose

   pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead Streptavidin-SpyTag
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   The construct lacks final K of SpyTag

   Promoter

   T7

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5FRT/TO-UBE2QL1-Strep/HA

   Plasmid

   #124666

   Purpose

   Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-FAF1-Strep-HA

   Plasmid

   #113486

   Purpose

   Expression of human FAF1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   FAF1 (FAF1 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fluorescent reporter for MOBE1-2

   Plasmid

   #219945

   Purpose

   Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   EGFP(A111V/L202S)

   Promoter

   CMV, U6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-surrogate-reporter

    Plasmid

    #65624

    Purpose

    Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attP
    

    Use

    Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

    Plasmid

    #129513

    Purpose

    Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 C88S (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-ccdB-TwinStrep

    Plasmid

    #214988

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS18 (mANGPTL3-Strep)

    Plasmid

    #109111

    Purpose

    Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (Angptl3 Mouse)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCDNA4.TO-ORF45-2xCSTREP

    Plasmid

    #136206

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF45

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF45
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5FRT/TO-VCPIP1-Strep-HA

    Plasmid

    #113482

    Purpose

    Expression of human VCPIP1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    VCPIP1 (VCPIP1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-PDGFRA-reporter

    Plasmid

    #136456

    Purpose

    Mammalian fluorescent reporter plasmid for PDGFRA signaling.

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Inserts

    PDGFRalpha (PDGFRA Human)
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and Minimal promoter with artificial SRE enha…

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRT/TO-p37-Strep-HA

    Plasmid

    #113485

    Purpose

    Expression of human p37 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p37 (UBXN2B Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5FRT/TO-UBXD4-Strep-HA

    Plasmid

    #113480

    Purpose

    Expression of human UBXD4 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD4 (UBXN2A Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4.TO-ORF65-2xCSTREP

    Plasmid

    #136225

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF65

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3F-Strep-mMALT1

    Plasmid

    #33315

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    Malt1 (Malt1 Mouse)
    

    Use

    Tags

    3xFLAG and StrepOne

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only