Skip to main content
Addgene
Showing: 421 - 440 of 4687 results

  3. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

    Enlarge

  7. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid
    #228576
    Purpose
    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    BRD4 gRNA (BRD4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228576-plasmid-map-sequence-id-457355

    Enlarge

  13. CMV:AsCpf1-2A-GFP-U6-tdTomato-sg

    Plasmid
    #194722
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomato
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194722-plasmid-map-sequence-id-381295

    Enlarge

  14. CMV:AsCpf1-2A-GFP-U6-INS-sg

    Plasmid
    #194719
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1 (INS Acidaminococcus sp.)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194719-plasmid-map-sequence-id-381224

    Enlarge

  15. CMV:AsCpf1-2A-GFP-U6-MAFB-sg

    Plasmid
    #194718
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1 (MAFB Acidaminococcus sp.)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194718-plasmid-map-sequence-id-381223

    Enlarge

  16. CMV:AsCpf1-2A-GFP-U6-GFP-sg

    Plasmid
    #194717
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194717-plasmid-map-sequence-id-381222

    Enlarge

  18. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

    Plasmid
    #159919
    Purpose
    Mutagenesis of Vamp1
    Depositor
    Larry Zweifel
    Insert
    Vamp1 (Vamp1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159919-plasmid-map-sequence-id-317336

    Enlarge

  19. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

    Plasmid
    #159911
    Purpose
    Mutagenesis of Kcnq3
    Depositor
    Larry Zweifel
    Insert
    Kcnq3 (Kcnq3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159911-plasmid-map-sequence-id-317318

    Enlarge

  20. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid
    #159907
    Purpose
    Mutagenesis of Netrin1
    Depositor
    Larry Zweifel
    Insert
    Netrin1 (Ntn1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159907-plasmid-map-sequence-id-317313

    Enlarge

Showing: 421 - 440 of 4687 results