Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 7,249 results for: ALP

Showing: 841 - 880 of 7249 results

  2. pS238D1::sptse5-CT_phoA-lacZalpha

    Plasmid
    #192961
    Purpose
    Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT_phoA-lacZ_
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1317) fused to phoA-lacZal…
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192961-plasmid-map-sequence-id-380611

    Enlarge

  6. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid
    #181891
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181891-plasmid-map-sequence-id-359705

    Enlarge

  8. pPD1024 EF1alpha TetON3G in TU5

    Plasmid
    #139261
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV5
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139261-plasmid-map-sequence-id-325374

    Enlarge

  9. pJM469 EF1alpha TetON3G in TU4

    Plasmid
    #139260
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV4
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139260-plasmid-map-sequence-id-325373

    Enlarge

  10. pJM422 EF1alpha TetON3G in TU3

    Plasmid
    #139259
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV3
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139259-plasmid-map-sequence-id-325372

    Enlarge

  13. HA-Clover-HIF-2alpha T406E

    Plasmid
    #163379
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Glu) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T406E
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163379-plasmid-map-sequence-id-322519

    Enlarge

  14. HA-Clover-HIF-2alpha T406A

    Plasmid
    #163378
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T406A
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163378-plasmid-map-sequence-id-322518

    Enlarge

  15. HA-Clover-HIF-2alpha S581D

    Plasmid
    #163377
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Asp) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S581D
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163377-plasmid-map-sequence-id-322517

    Enlarge

  16. HA-Clover-HIF-2alpha S581A

    Plasmid
    #163376
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S581A
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163376-plasmid-map-sequence-id-322516

    Enlarge

  17. HA-Clover-HIF-2alpha T528E

    Plasmid
    #163375
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr528Glu) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T528E
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163375-plasmid-map-sequence-id-322515

    Enlarge

  18. HA-Clover-HIF-2alpha T528A

    Plasmid
    #163374
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr528Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T528A
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163374-plasmid-map-sequence-id-322511

    Enlarge

  19. HA-Clover-HIF-2alpha S345D

    Plasmid
    #163373
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser345Asp) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S345D
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163373-plasmid-map-sequence-id-322298

    Enlarge

  20. HA-Clover-HIF-2alpha S345A

    Plasmid
    #163372
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser345Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S345A
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163372-plasmid-map-sequence-id-322296

    Enlarge

  21. HA-Clover-HIF-1alpha S31D

    Plasmid
    #163370
    Purpose
    Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Asp) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha S31D (HIF1A Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S31D
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163370-plasmid-map-sequence-id-322293

    Enlarge

  22. HA-Clover-HIF-1alpha S31G

    Plasmid
    #163369
    Purpose
    Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Gly) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha S31G (HIF1A Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S31G
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163369-plasmid-map-sequence-id-322292

    Enlarge

  23. HA-Clover-HIF-1alpha S786D

    Plasmid
    #163368
    Purpose
    Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Asp) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha S786D (HIF1A Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S786D
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163368-plasmid-map-sequence-id-322280

    Enlarge

  24. HA-Clover-HIF-1alpha S786A

    Plasmid
    #163367
    Purpose
    Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha S786A (HIF1A Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S786A
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163367-plasmid-map-sequence-id-322279

    Enlarge

  26. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

    Plasmid
    #160614
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160614-plasmid-map-sequence-id-322024

    Enlarge

  27. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

    Plasmid
    #160615
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160615-plasmid-map-sequence-id-322022

    Enlarge

  35. pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H

    Plasmid
    #108374
    Purpose
    Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, TNF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    108374-plasmid-map-sequence-id-214321

    Enlarge

  37. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 841 - 880 of 7249 results