We narrowed to 1,414 results for: PUC57
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Plasmid#216066PurposeFragmid fragment: (2A Selection) Neomycin resistanceDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; NeoR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA043
Plasmid#215964PurposeFragmid fragment: (Pol 2 promoter) human Phosphoglycerate Kinase promoterDepositorHas ServiceCloning Grade DNAInsertPGK_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA066
Plasmid#215966PurposeFragmid fragment: (Pol 2 promoter) human UbiquitinC promoterDepositorHas ServiceCloning Grade DNAInsertUbC_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA369
Plasmid#215987PurposeFragmid fragment: (N' terminus) attenuate expressionDepositorHas ServiceCloning Grade DNAInsertStart; u(S)ORF_v1.1; Stop; Start
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA181
Plasmid#216024PurposeFragmid fragment: (Cas protein) unmodified AsCas12a; not preferredDepositorHas ServiceCloning Grade DNAInsertCas12a_v1.1 [As]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA149
Plasmid#216060PurposeFragmid fragment: (2A Selection) Hygromycin resistanceDepositorHas ServiceCloning Grade DNAInsertT2A_v1.3; HygroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA365
Plasmid#215969PurposeFragmid fragment: (Pol 2 promoter) Gal4-induced expressionDepositorHas ServiceCloning Grade DNAInsertLoxP_v1; 5xUAS_v1; LoxP_v1; 5xUAS_v1; Hsp70_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA265
Plasmid#215967PurposeFragmid fragment: (Pol 2 promoter) spleen focus-forming virus promoterDepositorHas ServiceCloning Grade DNAInsertSFFV_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA371
Plasmid#215989PurposeFragmid fragment: (N' terminus) attenuate expressionDepositorHas ServiceCloning Grade DNAInsertStart; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA416
Plasmid#215992PurposeFragmid fragment: (N' terminus) luminescent reporterDepositorHas ServiceCloning Grade DNAInsertStart; V5_v1.1; NanoLuc_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA195
Plasmid#215984PurposeFragmid fragment: (N' terminus) ALFA tag for recruiting via NbALFADepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA198
Plasmid#215985PurposeFragmid fragment: (N' terminus) activator from p65 subunit of mouse NfkbDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; p65_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA125
Plasmid#215982PurposeFragmid fragment: (N' terminus) activator from p65 subunit of mouse NfkbDepositorHas ServiceCloning Grade DNAInsertStart; p65_v1.3; HSF1_v1.2; Linker_v3.1; SV40NLS_v1.6
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA081
Plasmid#215977PurposeFragmid fragment: (N' terminus) HIV Nuclear Export SequenceDepositorHas ServiceCloning Grade DNAInsertStart; HIV-NES_v1.2; HIV-NES_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA124
Plasmid#215981PurposeFragmid fragment: (N' terminus) catalytic core of human acetyltransferase p300DepositorHas ServiceCloning Grade DNAInsertStart; p300_v1.2; Linker_v3.1; SV40NLS_v1.6
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA141
Plasmid#216045PurposeFragmid fragment: (C' terminus) SV40NLS; Linker; p300DepositorHas ServiceCloning Grade DNAInsertSV40NLS_v1.7; Linker_v4.1; p300_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA122
Plasmid#215980PurposeFragmid fragment: (N' terminus) 4xVP16 transcritional activatorsDepositorHas ServiceCloning Grade DNAInsertStart; VP64_v1.1; Linker_v3.1; SV40NLS_v1.6
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA091
Plasmid#215979PurposeFragmid fragment: (N' terminus) ZNF10 KRABDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; KRAB_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA088
Plasmid#215978PurposeFragmid fragment: (N' terminus) ZIM3 KRABDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; KRAB_v2.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA334
Plasmid#216068PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; BlastR; StopDepositorHas ServiceCloning Grade DNAInsertStop; IRES2_v1; Start; BlastR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA151
Plasmid#215938PurposeFragmid fragment: (guide cassette) for dual expression sgRNADepositorHas ServiceCloning Grade DNAInsertU6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA375
Plasmid#216054PurposeFragmid fragment: (C' terminus) HIV nuclear export sequence; HA tagDepositorHas ServiceCloning Grade DNAInsertHIV-NES_v1.1; HIV-NES_v1.2; 3xHA_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA428
Plasmid#216055PurposeFragmid fragment: (C' terminus) NpNLS; 3xHA; c-mycNLS; c-mycNLSDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.1; 3xHA_v1.1; c-mycNLS_v1.2; c-mycNLS_v1.3
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA400
Plasmid#215958PurposeFragmid fragment: (guide cassette) guide expressionDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v1 [Rfx]; BsmBI_v1; BsmBI_v12
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA050
Plasmid#216059PurposeFragmid fragment: (2A Selection) violet-excited GFPDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; VexGFP_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA076
Plasmid#216021PurposeFragmid fragment: (Cas protein) Cas13dDepositorHas ServiceCloning Grade DNAInsertCas13d_v1.1 [Rfx]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA401
Plasmid#215959PurposeFragmid fragment: (guide cassette) guide expressionDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA012
Plasmid#215974PurposeFragmid fragment: (N' terminus) Bipartite Nuclear Localization SignalDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA370
Plasmid#215988PurposeFragmid fragment: (N' terminus) attenuate expressionDepositorHas ServiceCloning Grade DNAInsertStart; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Luciferase-CD4-bla
Plasmid#189981PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Halo-CD4-bla
Plasmid#189982PurposeDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-mScarlet-I-CD4-bla
Plasmid#189984PurposeDonor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Luciferase-CD4-bla
Plasmid#189985PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-GFP-T2A
Plasmid#203608PurposeDonor plasmid to create 5' knockin of NKX2-5 with GFP-T2ADepositorInsertNKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
UseHuman targetingTagsExpressionMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_A28C_R
Plasmid#200184PurposeWT PA14 I-F Leader and A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (A28C)
UseTagsExpressionBacterialMutationA28C in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1D
Plasmid#196686PurposeRep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTFR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1B
Plasmid#196684PurposeRep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPSQGTLR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1A
Plasmid#196683PurposeRep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTVR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
fru-T2A-QF2 HDR plasmid
Plasmid#141099PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru geneDepositorInsertfru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1043
Plasmid#169621PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1042
Plasmid#169620PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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FLAG-LoxP-PURO-LoxP-SNAP-TERT
Plasmid#71390PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.DepositorInsertTERT (TERT Human)
UseHr donorTagsFLAG-SNAPExpressionMutationPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞MCS
Plasmid#154013PurposeExpression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoterDepositorInsertMCS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞GFP
Plasmid#154015PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposaseDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞RFP
Plasmid#154016PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationH210PPromoterCAGAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.V1
Plasmid#127847Purposenon-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.V1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only