Addgene

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pSC-cUnaG

Plasmid

#207631

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-cUnaG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ARP3-V5-TurboID

Plasmid

#195144

Purpose

measure COR1C interaction boichemically

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

ACTR3 (ACTR3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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COR1C CC-Halo

Plasmid

#195146

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

truncated protein

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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XLone-GvpB

Plasmid

#173798

Purpose

Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpB

Use

Tags

mCherry (K187N -please see depositor comments)

Expression

Mammalian

Mutation

Promoter

TRE3G promoter

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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HySp5:Luc

Plasmid

#192985

Purpose

Plasmid to test the Hydra Sp5 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfWnt3:Luc

Plasmid

#192992

Purpose

Plasmid to test the Zebrafish Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLZRS-panTRK-CR1-EGFP

Plasmid

#187194

Purpose

To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3

Depositor

Pantelis Tsoulfas

Article

O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7.

Insert

CR1 pan-TRK receptor

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

mo-MLV or MMLV

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA

Plasmid

#186396

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DPB

Plasmid

#164989

Purpose

Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPB1*01:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DQB

Plasmid

#164991

Purpose

Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DQB1*05:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DRB

Plasmid

#164992

Purpose

Expression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DRB1*01:02:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DQA

Plasmid

#164990

Purpose

Expression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DQA1*01:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DPA

Plasmid

#164988

Purpose

Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPA1*02:01:08

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

Plasmid

#73542

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

Plasmid

#73543

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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L1-AA027

Plasmid

#185752

Purpose

Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

HygR2

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

Plasmid

#170768

Purpose

Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Inserts

M.XhoI
M.MunI
M.Ocy1ORF8430P

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pIIS18-BufAI-2

Plasmid

#128651

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIIS18-BufAI-1

Plasmid

#128650

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-Cerulean

Plasmid

#127771

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

ednrbE2

Use

Enhancer reporter construct

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE1-Citrine

Plasmid

#127773

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

ednrbE1

Use

Enhancer reporter construct

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Sox3gRNA-T2

Plasmid

#127778

Purpose

Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox3gRNAT2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX 4T-1/MKRN1

Plasmid

#78757

Purpose

To purify GST-tagged MKRN1 recombinant protein

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MKRN1 110-482

Plasmid

#78747

Purpose

To overexpress MKRN1 110-482 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

110-482

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MKRN1 1-280

Plasmid

#78750

Purpose

To overexpress -MKRN1 1-280 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

1-280

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1 N297A

Plasmid

#66723

Purpose

Constitutive expression of FPR1 N297A in mammalian cells.

Depositor

Heini Miettinen

Article

Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

Insert

FPR1 (haplotype 3) (FPR1 Human)

Use

Tags

Expression

Mammalian

Mutation

N297A

Promoter

SR-alpha

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 S334A

Plasmid

#51593

Purpose

expresses RNAiR myc-tagged Rem2 S-A mutant

Depositor

Suzanne Paradis

Article

Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Serine 334 to Alanine; also RNAi resistant

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pUC:FCP:ShBle:FCP:EGFP

Plasmid

#85987

Purpose

Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

Depositor

Thomas Mock

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Inserts

ShBle
EGFP

Use

Tags

Expression

Mutation

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-16

Plasmid

#160716

Purpose

T-nos Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

nopaline synthase terminator (T-nos) bordered by BsaI restriction sites

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSIV-D3psi/delta env/delta Vif/delta Vpr

Plasmid

#132928

Purpose

Production of SIV-VLPs containing Vpx protein

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

Gag, pol, and vpx

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Article

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

8X Gli-binding site

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-TetOne-Puro-mitoLbNOX-FLAG

Plasmid

#218667

Purpose

pLVX-TetOne-Puro-mitoLbNOX-FLAG

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

mitoLbNOX

Use

Lentiviral and Synthetic Biology

Tags

FLAG and MTS

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-TetOne-Puro-LbNOX-FLAG

Plasmid

#218631

Purpose

This plasmid contains LbNOX under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LbNOX

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Tags

Expression

Mutation

Promoter

opposing T7 promoters

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CamKIIa_GFP

Plasmid

#96941

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

Green fluorescent protein

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1.ARRDC1

Plasmid

#38320

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPERIOR.puro-shp53

Plasmid

#38035

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shp53 (TP53 Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
∆DUSP6

Plasmid

#27977

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

C293S: mutation in the phosphatase domain

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
SiRES COR1C-GFP

Plasmid

#195154

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHC005 (∆RE)

Plasmid

#226199

Purpose

Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for type I restriction-modification system restriction subunit H16_A0006

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2-Beclin1

Plasmid

#99574

Purpose

Expressed Cas9 with sgRNA targeting Beclin1

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting Beclin1 (BECN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-fNPY-GFP

Plasmid

#22912

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu neuropeptide Y promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-Blast coUGP2

Plasmid

#211531

Purpose

Expresses codon optimized UGP2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

UGP2 (UGP2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
p5M-hPR-B

Plasmid

#89114

Purpose

mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Use

Tags

Expression

Mammalian

Mutation

5 restriction sites deleted in polylinker

Promoter

CMV

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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