We narrowed to 607 results for: Anti-CRISPR
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Plasmid#188498PurposeExpresses FLAG tagged Cas12j2DepositorInsertCas12j2
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX458-gR2A_ISO
Plasmid#124808PurposeVector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.DepositorInserthSPCas9
Tags3XFLAG and GFPExpressionMammalianAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e KRAB
Plasmid#188506PurposeExpresses FLAG tagged PlmdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-KRAB-NLSExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1_Cas9-Ck4
Plasmid#136135PurposeL1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectorsDepositorInsertp5-MpEF1a:Cas9_3tNos-35S
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G1
Plasmid#188963PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: atcggtcgcattgttttccactagg
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianPromoterCAGAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNTI763 p(Z)-mCherry + UBC9 sgRNA
Plasmid#164918PurposeCEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNADepositorInsertP(Z)-mcherry
UseCRISPRExpressionYeastPromoterP(Z), synthetic promoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG3-srt-his
Plasmid#124805PurposeHDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.DepositorInsertMurine IgG3
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgApc/Cre
Plasmid#89641PurposeExpresses an Apc-targeting gRNA and Cre-recombinaseDepositorAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_P278A
Plasmid#183113PurposeExpresses flag-tagged p53 with P278A mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged proline 278 to alanine, see depositor com…PromoterEF1aAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1_lacZgRNA-Ck3
Plasmid#136136PurposeL1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screeningDepositorInsertp5-MpU6:lacZgRNA
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgArid1a/Cre
Plasmid#89642PurposeExpresses an Arid1a-targeting gRNA and Cre-recombinaseDepositorAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNT3/Cre
Plasmid#89654PurposeExpresses a non-targeting gRNA and Cre-recombinaseDepositorInsertnon-targeting gRNA
UseCre/Lox and LentiviralExpressionMammalianAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1_lacZgRNA-Ck2
Plasmid#136137PurposeL1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screeningDepositorInsertp5-MpU6:lacZgRNA
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRb1/Cre
Plasmid#89647PurposeExpresses an Rb1-targeting gRNA and Cre-recombinaseDepositorAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEW100v5-PUR-T7-sgRNA
Plasmid#245108PurposePlasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. bruceiDepositorTypeEmpty backboneUseCRISPRAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-gR082
Plasmid#188553PurposeBinary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)DepositorInsertgR082
UseCRISPR and Gateway CloningAvailable SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEW100v5-NEO-T7-sgRNA
Plasmid#245118PurposePlasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. bruceiDepositorTypeEmpty backboneUseCRISPRAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGMC00014
Plasmid#172526Purposenon targeting sgRNADepositorInsertNon-targeting guide
UseLentiviralExpressionMammalianAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY113 (pcDNA3.1-huPb2Cpf1)
Plasmid#92272PurposeExpression of humanized Pb2Cpf1DepositorInserthuPb2Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only