We narrowed to 833 results for: Anti-CRISPR

1. CDS12_Cas9-NLS

   Plasmid

   #136122

   Purpose

   Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_AtcoCas9-NLS(noStop)
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-Lck

   Plasmid

   #159430

   Purpose

   CRISPR knockout of Lck

   Depositor

   Arthur Salomon

   Article

   Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

   Insert

   sgRNA targeting Lck exon 2 (LCK Human)
   

   Use

   CRISPR

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-gR2A_ISO

   Plasmid

   #124808

   Purpose

   Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

   Depositor

   Martijn Verdoes, Ferenc Scheeren

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   hSPCas9
   

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ-dpcoCas9-SunTag

   Plasmid

   #158410

   Purpose

   CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGWBhis03-Citrine

   Plasmid

   #188557

   Purpose

   Binary vector for the expression of Citrine with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   Citrine and MpIGPDm
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMpGE_En03-gR082

   Plasmid

   #188551

   Purpose

   Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   gR082
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMpGE_En03-gR085

   Plasmid

   #188552

   Purpose

   Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   gR085
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMpGWBhis03-Citrine-NLS

   Plasmid

   #188558

   Purpose

   Binary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   Citrine-NLS and MpIGPDm
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-scFv-VP64_Blast

   Plasmid

   #192655

   Purpose

   3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   scFv-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry Codon 59 sgRNA

    Plasmid

    #109431

    Purpose

    MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry codon 59 gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY279

    Plasmid

    #182959

    Purpose

    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Insert

    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDY281

    Plasmid

    #182960

    Purpose

    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Insert

    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ-dzCas9-Act3.0

    Plasmid

    #158414

    Purpose

    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ-dSpRY-Act3.0

    Plasmid

    #158416

    Purpose

    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ-dpcoCas9-Act3.0

    Plasmid

    #158408

    Purpose

    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. INT_SS9sp

    Plasmid

    #212145

    Purpose

    Effector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC
    

    Expression

    Bacterial

    Promoter

    pJ23119

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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