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Mixed-signal integration BAC Reporter

Plasmid

#78223

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K1S

Plasmid

#62979

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 1-446 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pISlin41m(s) (lin-41 microRNA mut reporter)

Plasmid

#11907

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence mut (lin-41 Nematode)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

point mutations in miRNA complementary site

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-mir-40

Plasmid

#46739

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-40

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir17~20

Plasmid

#46671

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir17~20 (MIR17 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1.2

Plasmid

#46681

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.2 (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-mir-2a-1

Plasmid

#46664

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-2a-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)

Plasmid

#232411

Purpose

AAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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SEAP (Protein secretion reporter) in pLX317

Plasmid

#233290

Purpose

SEAP reporter (used for monitoring protein secretion)

Depositor

Sefi Rosenbluh

Insert

SEAP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT375: pDonor(ZooTsy)_excision-reporter

Plasmid

#200873

Purpose

Reporter for ZooTsy excision.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT (PGK) 12xHRE-NanoLuc

Plasmid

#208575

Purpose

hypoxic response element driven NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

NanoLuciferase
d2eGFP
12xHRE

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR

Plasmid

#211820

Purpose

Lentiviral vector expressing a dead GFP adenine base editing reporter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

dead GFP reporter

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter and SV40 promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-CDK5RAP2-SVA

Plasmid

#202828

Purpose

pGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

intronic SVA within CDK5RAP2 gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-AGPAT3-SVA

Plasmid

#202827

Purpose

pGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

intronic SVA located in AGPAT3 gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-JAM2-SVA

Plasmid

#202832

Purpose

pGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

JAM2 intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-POFUT2-SVA

Plasmid

#202831

Purpose

pGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

POFUT2 intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-HTT-SVA

Plasmid

#202833

Purpose

pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

HTT intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-CHAF1B-SVA

Plasmid

#202829

Purpose

pGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

CHAF1B intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK/CMV)-GRE

Plasmid

#172336

Purpose

Gal4 Response element

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Mutation

Promoter

PGK/CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

Plasmid

#172598

Purpose

Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.

Depositor

Daniel Kiss

Article

Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

Insert

myc-PDGFRA-D842V (PDGFRA Synthetic, Human)

Use

Egfp-pest_reporter

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

PDGFRA-D842V

Promoter

CMV

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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