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CLTC G9.4 gRNA

Plasmid

#90634

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLTC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CLTC (Guide Designation G9.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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BRD4 CRISPRi plasmid

Plasmid

#154890

Purpose

Human BRD4 CRISPRi gRNA

Depositor

Rob Mitra, Joseph Dougherty

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

BRD4-targeting gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR/U6 HDAC5 shRNA

Plasmid

#32222

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against mouse HDAC5 (Hdac5 Mouse)

Use

RNAi; Gateway u6 entry vector

Tags

Expression

Mutation

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
Tol2-LyzC-RacFRET-U6ac-rac2 guides

Plasmid

#168248

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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DHCR7 B5.3 gRNA

Plasmid

#90650

Purpose

3rd generation lentiviral gRNA plasmid targeting human DHCR7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DHCR7 (Guide Designation B5.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBS/U6/RNAi gamma-tubulin shRNA mouse

Plasmid

#70666

Purpose

Reduced the expression of gamma-tubulin in murine cells

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)

Use

U6 promoter

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCAS9n(BB)-2A-puro DNM1, B

Plasmid

#107796

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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PPP1CB C3.5 gRNA

Plasmid

#90845

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP1CB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP1CB (Guide Designation C3.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

Plasmid

#138990

Purpose

IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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CDC6 B5.1 gRNA

Plasmid

#90596

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B5.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128342

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna7 (Chrna7 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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CDC6 B6.1 gRNA

Plasmid

#90597

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B6.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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RRM1 E2.3 gRNA

Plasmid

#90881

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM1 (Guide Designation E2.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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MASTL F3.1 gRNA

Plasmid

#90752

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation F3.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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CSNK1A1 H1.4 gRNA

Plasmid

#90640

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CSNK1A1 (Guide Designation H1.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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gh173

Plasmid

#106834

Purpose

expression of gRNA targeting UGGT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGGT2 (UGGT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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beta4-nAChR shRNA (Beta-4.3)

Plasmid

#86650

Purpose

expression of shRNA targeting CHRNB4

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (beta4-nAChR) (CHRNB4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Px458-Cas9n-Trp73-Exon4-4sgRNA

Plasmid

#88851

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73 (Trp73 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
FgH1tUTG_huMcl-1.2

Plasmid

#85531

Purpose

Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TPX2 H2.2 gRNA

Plasmid

#90919

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPX2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TPX2 (Guide Designation H2.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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