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Plasmid#188557PurposeBinary vector for the expression of Citrine with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .DepositorInsertCitrine and MpIGPDm
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-gR082
Plasmid#188551PurposeGateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.DepositorInsertgR082
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-gR085
Plasmid#188552PurposeGateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.DepositorInsertgR085
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
p70rg 11
Plasmid#17830DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-NT*-HRV3CP
Plasmid#162795PurposeExpressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)DepositorInsertHuman rhinovirus 14 3C protease
UseTagsNT* solubility tagExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianMutationPromoterMSSV_LTRAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC
Plasmid#221134PurposeContains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminatorDepositorInserts[PibpA-gfpASV]
[PcpxPmcherryASV] OPT
UseTagsExpressionBacterialMutationPromotercpxP and ibpAAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIKU801-CrPEPC1
Plasmid#92342PurposeExpresses sgRNA for targeting CrPEPC1 gene, paromomycin resistanceDepositorInsertsgRNA-CrPEPC1
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-67Sma
Plasmid#49342PurposeChlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic regionDepositorInsertEcoRI 5.8 kB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
ykgMO-IGS-sfGFP
Plasmid#207231PurposeCalprotectin sensing promoter with sfGFP reporterDepositorInsertykgMO-IGS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRF2-IRESS-eGFP
Plasmid#19798DepositorInsertTRF2 (TERF2 Human)
UseTagsExpressionMammalianMutationWhen compared to GenBank reference sequence NP_00…PromoterCMV-FAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ECFPEYFP
Plasmid#24520DepositorInsertECFP, EYFP
UseTagsECFP and EYFPExpressionMammalianMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
6xO/S luc
Plasmid#69445Purposedetection/assessment of OCT4 and SOX2 transcription factor activityDepositorInsertminimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHdzCas9-KRAB
Plasmid#92341PurposeExpression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistanceDepositorTypeEmpty backboneUseCRISPR; Plant expressionTagsKRABExpressionPlantMutationPromoterCaMV35SAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03
Plasmid#67137PurposeGateway destination vector for cell-free expression with N-term 8xHis eGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G01
Plasmid#67135PurposeGateway destination vector for cell-free expression with N-term 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA180
Plasmid#128538PurposeExpresses E coli Bir A proteinDepositorInsertE coli BirA
UseTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G04
Plasmid#67138PurposeGateway destination vector for cell-free expression with C-term eGFP 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HISExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only