We narrowed to 18,833 results for: Tec

1. pMpGE010-gR085

   Plasmid

   #188554

   Purpose

   Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   gR085
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMpGWBhis03-Citrine

   Plasmid

   #188557

   Purpose

   Binary vector for the expression of Citrine with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   Citrine and MpIGPDm
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGE_En03-gR082

   Plasmid

   #188551

   Purpose

   Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   gR082
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGE_En03-gR085

   Plasmid

   #188552

   Purpose

   Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   gR085
   

   Use

   CRISPR; Entry vector

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p70rg 11

   Plasmid

   #17830

   Depositor

   Jay Keasling

   Article

   Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

   Insert

   E. coli optimized red flourescent protein (RFPec), GFPuv
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pET-NT*-HRV3CP

   Plasmid

   #162795

   Purpose

   Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

   Depositor

   Gottfried Otting

   Article

   Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

   Insert

   Human rhinovirus 14 3C protease
   

   Tags

   NT* solubility tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-M2

   Plasmid

   #135583

   Purpose

   High eukaryotic protein expression shuttle vector

   Depositor

   Xiao-Wen Cheng

   Article

   Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

   Type

   Empty backbone

   Use

   Transfer vector

   Expression

   Insect

   Promoter

   polyhedrin of AcMNPV

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-MBP-mSA2

   Plasmid

   #52319

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Tags

   FLAG, His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHdzCas9-KRAB

    Plasmid

    #92341

    Purpose

    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

    Depositor

    I-Son Ng

    Article

    Kao et al Bioresour Technol. 2017 May 4. pii: S096

    Type

    Empty backbone

    Use

    CRISPR; Plant expression

    Tags

    KRAB

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRMPVIR

    Plasmid

    #27994

    Purpose

    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TRMPVIR (shRen)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRF2-IRESS-eGFP

    Plasmid

    #19798

    Depositor

    Mark Mattson

    Article

    van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

    Insert

    TRF2 (TERF2 Human)
    

    Expression

    Mammalian

    Mutation

    When compared to GenBank reference sequence NP_00…

    Promoter

    CMV-F

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

13. TtRMPVIR

    Plasmid

    #27995

    Purpose

    Retroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains DsRed2 and Venus fluorescent reporters.

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TtRMPVIR (shRen)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEXP1/Joseph2-Multitagged Plasmid

    Plasmid

    #226724

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pEXP1/Joseph2
    

    Tags

    6xHis, HA, Myc, GFP, Flag, V5

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIKU801-CrPEPC1

    Plasmid

    #92342

    Purpose

    Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

    Depositor

    I-Son Ng

    Article

    Kao et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    sgRNA-CrPEPC1
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6-26

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ykgMO-IGS-LuxCDABE

    Plasmid

    #207232

    Purpose

    Calprotectin sensing promoter with luciferase reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xO/S luc

    Plasmid

    #69445

    Purpose

    detection/assessment of OCT4 and SOX2 transcription factor activity

    Depositor

    Lisa Dailey

    Insert

    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ykgMO-IGS-sfGFP

    Plasmid

    #207231

    Purpose

    Calprotectin sensing promoter with sfGFP reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQC

    Plasmid

    #221134

    Purpose

    Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator

    Depositor

    Daniel Daley

    Article

    Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102

    Inserts

    [PibpA-gfpASV]
    [PcpxPmcherryASV] OPT
    

    Expression

    Bacterial

    Promoter

    cpxP and ibpA

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-ECFPEYFP

    Plasmid

    #24520

    Depositor

    Jehonathan Pinthus, Liusheng He

    Article

    He et al Cytometry A. 2003 Oct . 55(2):71-85.

    Insert

    ECFP, EYFP
    

    Tags

    ECFP and EYFP

    Expression

    Mammalian

    Available Since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only