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EW387 (ZNF35/IKZF1tar-6bp spacers)x4-H2B-GFP

Plasmid

#236138

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNFIKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

E1b minimal promoter with four ZNF35/ZNFIKZF1 bin…

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEW107

Plasmid

#232796

Purpose

COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab

Depositor

Cynthia Wolberger

Article

Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

Insert

COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)

Use

Tags

His6-3xFLAG, Twinstrep

Expression

Insect

Mutation

ySet1(762-1080)

Promoter

polyhedrin promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_1bp-insertion

Plasmid

#201977

Purpose

Express a pegRNA used for 1bp insertion at HEK4 site

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_1bp-insertion

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TAB-mOvol1

Plasmid

#120360

Purpose

piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

Ovol1 (Ovol1 Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tet-on and CBh

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

Plasmid

#107300

Purpose

Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

Depositor

Scot Wolfe

Insert

Truncated SpCas9 (aa 574-1368) fused to FKBP

Use

CRISPR

Tags

3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

Expression

Mammalian

Mutation

Truncated SpCas9 (aa 574-1368) with R1335K mutati…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

Plasmid

#107299

Purpose

Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

Depositor

Scot Wolfe

Insert

Truncated SpCas9 (aa 574-1368) fused to FKBP

Use

CRISPR

Tags

3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

Expression

Mammalian

Mutation

Truncated SpCas9 (aa 574-1368) with R1335K mutati…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TAC-WTSI

Plasmid

#80486

Purpose

piggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

WTSI

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-PLXNB2

Plasmid

#69240

Purpose

U6 driven SpCas9 sgRNA expression for PLXNB2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

PLXNB2 sgRNA (PLXNB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

Plasmid

#69221

Purpose

Expresses R1333K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Lysine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

Plasmid

#69222

Purpose

Expresses R1333S mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Serine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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VPX-mRNA

Plasmid

#227679

Purpose

plasmid for VPX mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

VPX (HIV2gp3 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDACR3883 [rab-3p::HYlight-RA::let-858utr]

Plasmid

#207667

Purpose

Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

Depositor

Daniel Colón-Ramos

Article

Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

HYlight-RA [T152E]

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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p11d-aRPA (MSW#712)

Plasmid

#208078

Purpose

Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PEmax_2star_pet21A

Plasmid

#227675

Purpose

Expression plasmid for PEMAX** protein in bacteria.

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

PEmax_2_star

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFPluc2

Plasmid

#80493

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

GFPluc2

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PEmax_2star_IVT

Plasmid

#227674

Purpose

plasmid for PEmax** mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

PEmax_2_star

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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SauKKHCas9 PE2*

Plasmid

#169852

Purpose

Mammalian Expression, sauCas9-KKH based prime editor

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SaCas9KKH-N580A-M-MLV

Use

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Mutation

Promoter

cmv

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107320

Purpose

Expresses SpCas9 fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to SaCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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