We narrowed to 49,922 results for: des.1

1. pAP2042-1

   Plasmid

   #105244

   Purpose

   mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBKT7_AvrSr62-1

   Plasmid

   #233531

   Purpose

   Expresses AvrSr62-1 in yeast

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   AvrSr62-1
   

   Use

   Tags

   GAL4 BD, Myc tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 185_pETcon_SARS2_BQ1-1

   Plasmid

   #208085

   Purpose

   yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

   Depositor

   Tyler Starr

   Article

   Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

   Insert

   SARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFAP4_BHLH_5-1

   Plasmid

   #185054

   Purpose

   Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   TFAP4_5_1_gRNA (Tcfap4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. BB1_12_pFBA1-1

   Plasmid

   #98505

   Purpose

   BB1 with insert: promoter PFBA1-1

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   FBA1-1 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORTMAGE-1

   Plasmid

   #72680

   Purpose

   Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   lower strength RBS before mutL E32K allele than i…

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGEMHE:mTREK-1

   Plasmid

   #224743

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pS44i8GH-1

   Plasmid

   #207525

   Purpose

   Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3XHalo_GFP_TRF1#1

    Plasmid

    #164644

    Purpose

    tag TRF1 with GFP and Halo

    Depositor

    Michael Lampson, Huaiying Zhang

    Article

    Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1E-1

    Plasmid

    #21193

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (Esr1, MAP2K1 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Mek1 R4F fused to ER*, constitutive active

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. sgp27#1

    Plasmid

    #102759

    Purpose

    Expresses sgRNA targeting mouse p27

    Depositor

    Jin Chen

    Article

    Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. M2E-1

    Plasmid

    #21206

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek2 (Esr1, MAP2K2 Human, Mouse)
    

    Use

    Subclone vector

    Tags

    Expression

    Mutation

    deletion of residues EQQKKRLE, S222D S226D in Mek…

    Promoter

    Available since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pAP1791-1

    Plasmid

    #105246

    Purpose

    human eGFP with homology arms for RAB11A (recoded )

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    eGFP with homology arms for RAB11A (recoded *) (RAB11A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSPgRNA_BLK_E2-1

    Plasmid

    #124474

    Purpose

    For CRISPR-mediated epigenome editing of human BLK gene locus

    Depositor

    Iouri Chepelev

    Article

    Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

    Insert

    BLK_E2-1_gRNA (BLK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. shFGF2#1

    Plasmid

    #157667

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. shAPI5#1

    Plasmid

    #157665

    Purpose

    Tet inducible knockdown of API5

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO_FOXA1_#1

    Plasmid

    #70095

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO_HOXB13_#1

    Plasmid

    #70093

    Purpose

    Expression of shRNA to human HOXB13, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. lentiCRISPRv2_RB1#1

    Plasmid

    #174150

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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