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p236_LTJ_sgRNAFoxp3sf/J

Plasmid

#82676

Purpose

sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 sf/J

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p240_LTJ_sgRNAFoxp3K276X

Plasmid

#82675

Purpose

sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 K276X

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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p204_LTJ_sgRNACD45.2_R2

Plasmid

#82673

Purpose

sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p163_LTJ_sgRNACD90.1

Plasmid

#82677

Purpose

sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD90.1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p206_LTJ_sgRNACD45.2_R3

Plasmid

#82674

Purpose

sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AY23_pgRNA.R5.2

Plasmid

#100295

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6 Polymerase III snRNA

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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mCBFKOgRNA5

Plasmid

#84366

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
mCBFKOgRNA2

Plasmid

#84365

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pRPR1_g2gRNA_RPR1t

Plasmid

#64388

Purpose

encodes g2 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g2 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

pRPR1

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c2gRNA_RPR1t

Plasmid

#64380

Purpose

encodes c2 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c2 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_g1gRNA_RPR1t

Plasmid

#64387

Purpose

encodes g1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g1 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c7gRNA_RPR1t

Plasmid

#64385

Purpose

encodes c7 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c7 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c1gRNA_RPR1t

Plasmid

#64379

Purpose

encodes c1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c1 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c5gRNA_RPR1t

Plasmid

#64383

Purpose

encodes c5 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c5 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
MsMUT_sgRNA1

Plasmid

#111296

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine MUT

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA3

Plasmid

#111297

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA3 against murine MUT

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

Plasmid

#197029

Purpose

AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized ecDHFR-enOsCas12f1-ecDHFR

Use

AAV

Tags

Expression

Mutation

D52R / T132R

Promoter

MHCK7, hU6

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-p15

Plasmid

#62656

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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