We narrowed to 15,735 results for: LEA

1. SpG

   Plasmid

   #198543

   Purpose

   Expresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Golgi-deltaCKAR

   Plasmid

   #222428

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Golgi delta-selective C kinase activity reporter
   

   Tags

   CFP and YFP

   Expression

   Mammalian

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbA5c-cdl

   Plasmid

   #207649

   Purpose

   Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

   Depositor

   Benjamin Woolston

   Article

   Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

   Insert

   cdl
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYC-cdl

   Plasmid

   #207652

   Purpose

   Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

   Depositor

   Benjamin Woolston

   Article

   Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

   Insert

   cdl
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDLterm

   Plasmid

   #211903

   Purpose

   Base plasmid for dual-luciferase constructs to measure terminator strength

   Depositor

   Stan Fields

   Article

   Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Plant

   Promoter

   35S enhancer + 35S minimal promoter

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-HGM (optimized)

   Plasmid

   #220988

   Purpose

   Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

   Depositor

   Joshua Kritzer

   Article

   Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

   Insert

   HaloTag-GFP-mito
   

   Use

   AAV

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-U6.3-sgRNA-non-targeting-control -GFP

   Plasmid

   #221844

   Purpose

   Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

   Depositor

   Stephen Terry

   Article

   O’Sullivan et al bioRxiv 2024.03.04.583298

   Inserts

   EGFP
   non-targeting control sgRNA- GCACTGCTACGATCTACACC
   

   Use

   CRISPR; Tol2 transposon optimised for chick expre…

   Expression

   Mammalian

   Promoter

   GACG and U6.3 chick

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGK43

   Plasmid

   #222223

   Purpose

   Edits NPAS2 Gene.

   Depositor

   Seth Shipman

   Article

   Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTP489

   Plasmid

   #216366

   Purpose

   xNup155-Nb1t (+3Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xNup155-Nb1t (+3Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSC263

    Plasmid

    #216367

    Purpose

    xhNup155-Nb2i Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb2i
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-Avi-SUMOEu1

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSC261

    Plasmid

    #216371

    Purpose

    xhNup155-Nb3i Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb3i
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-Avi-SUMOEu1

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTP487

    Plasmid

    #216353

    Purpose

    xNup62-Nb1t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup62-Nb1t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP416

    Plasmid

    #216355

    Purpose

    xNup93-Nb1t (+1Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup93-Nb1t (+1Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSC262

    Plasmid

    #216359

    Purpose

    xhNup93-Nb4i Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup93-Nb4i
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-Avi-SUMOEu1

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSC233

    Plasmid

    #216360

    Purpose

    xhNup93-Nb4i (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup93-Nb4i (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSC264

    Plasmid

    #216363

    Purpose

    xhNup98-Nb2i Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup98-Nb2i
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-Avi-SUMOEu1

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGGA-pTRE-B

    Plasmid

    #218894

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    TRE
    

    Use

    Synthetic Biology

    Mutation

    580G>C

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGF-bGH polyA-G

    Plasmid

    #218902

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    bGH polyA
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGF-WPRE-G

    Plasmid

    #218901

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    WPRE
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGE-V5_TurboID-F

    Plasmid

    #218900

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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