We narrowed to 18,280 results for: Met;

1. mRuby2-Farnesyl-5

   Plasmid

   #55896

   Purpose

   Localization: Membrane, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCDF-Az

   Plasmid

   #197101

   Purpose

   To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAWG-dCas9-VPR

   Plasmid

   #63802

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Insect

   Mutation

   Promoter

   act5c

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-VTKS4

   Plasmid

   #170855

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.2/V5-DEST MTFR1L-V5

   Plasmid

   #69822

   Purpose

   Expresses MTFR1L-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Mitochondrial fission regulator 1-like (MTFR1L Human)
   

   Use

   Retroviral

   Tags

   v5

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. DRH322: AAV-CaMKIIa-QuasAr2-mO2

   Plasmid

   #51696

   Purpose

   AAV production of QuasAr2 fluorescent reporter

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr2-mOrange
   

   Use

   AAV

   Tags

   mOrange2

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTetON-DeAct-SpvB

   Plasmid

   #89455

   Purpose

   Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   TRE3G

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-REX-GECO1

   Plasmid

   #61248

   Purpose

   Expresses REX-GECO1 in neurons

   Depositor

   Robert Campbell

   Article

   Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

   Insert

   REX-GECO1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: P60R, V61W, R6…

   Promoter

   hSyn

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-FLEX-rc[Chronos-GFP]

   Plasmid

   #59056

   Purpose

   Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

    Plasmid

    #182412

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-hGH (GH1 Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-VTKS5

    Plasmid

    #170856

    Purpose

    AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid

    #89154

    Purpose

    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    Intein and chitin binding domain

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOS010: pLX304-CheRiff-GFP

    Plasmid

    #62986

    Purpose

    Lentivirus production of CheRiff channelrhodopsin actuator

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    CheRiff-eGFP
    

    Use

    Lentiviral; Gateway destination vector

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mIFP12-CD81-10

    Plasmid

    #56248

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    CD81 (CD81 Human)
    

    Use

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pAAV-VTKD5-GCaMP6f

    Plasmid

    #178728

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY036_ATP1A1_G5_Array

    Plasmid

    #86620

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Str-STIM1-NN_puromycin

    Plasmid

    #65310

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Use

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-VTKS6

    Plasmid

    #170857

    Purpose

    AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMOS009: pLX304-QuasAr2

    Plasmid

    #62985

    Purpose

    Lentivirus production of QuasAr2 fluorescent reporter

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr2-mOrange
    

    Use

    Lentiviral; Gateway destination vector

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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