We narrowed to 13,804 results for: TIM

1. PIP5K1A gRNA (BRDN0001147420)

   Plasmid

   #77964

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1A (PIP5K1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PIP5K1B gRNA (BRDN0001147624)

   Plasmid

   #77083

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1B (PIP5K1B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. INCTbiosyn-pAct-AtINT4

   Plasmid

   #127530

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase 4.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 4 coding sequence codon optimized for A. thaliana expression.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-HA-FbxO10-pcDNA3.1-

   Plasmid

   #52508

   Purpose

   expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO10 (FBXO10 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-dHeFSpCas9-VP64-6xHis

   Plasmid

   #92119

   Purpose

   Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. sTR056

   Plasmid

   #177369

   Purpose

   Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

   Depositor

   Jean-Loup Faulon, Jerome Bonnet

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. sTR060

   Plasmid

   #177370

   Purpose

   Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

   Depositor

   Jean-Loup Faulon, Jerome Bonnet

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   Trigger RNA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-45b-NFYC-IDR-mEGFP

   Plasmid

   #185015

   Purpose

   Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   NFYC IDR (Nfyc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NUAK1 gRNA (BRDN0001145557)

   Plasmid

   #75575

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NUAK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NUAK1 (NUAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ROCK2 gRNA (BRDN0001146481)

    Plasmid

    #76350

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. non-targeting control gRNA (BRDN0001145269)

    Plasmid

    #80249

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. T7T3-PacI-elfn1_459to1199

    Plasmid

    #87671

    Purpose

    In situ probe for mouse Elfn1

    Depositor

    Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    Elfn1 (Elfn1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    partial sequence, (459 to 1199bp off NM_175522.3)

    Promoter

    T7/T3

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176135

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

    Plasmid

    #174642

    Purpose

    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-Venus

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PIP5K1A gRNA (BRDN0001487080)

    Plasmid

    #77962

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PIP5K1B gRNA (BRDN0001146307)

    Plasmid

    #77085

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1B (PIP5K1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PIP5K1B gRNA (BRDN0001148929)

    Plasmid

    #77086

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1B (PIP5K1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. non-targeting control gRNA (BRDN0001148372)

    Plasmid

    #80258

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

    Plasmid

    #133788

    Purpose

    CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized St3Cas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PRPS2 gRNA (BRDN0001147559)

    Plasmid

    #78005

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRPS2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRPS2 (PRPS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge