We narrowed to 14,039 results for: TIM

1. Cox7c-pHLuorin-sems

   Plasmid

   #107766

   Purpose

   labeling of mitochondrial cytochrome c oxidase, pH measurements

   Depositor

   Karin Busch

   Article

   Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

   Insert

   Subunit Cox7c fused to pHluorin (COX7C Human)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ1320

   Plasmid

   #84829

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Expression

   Bacterial and Worm

   Promoter

   Peft-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJR106

   Plasmid

   #187244

   Purpose

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
   

   Use

   Lentiviral

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MYH11-gRNA1

   Plasmid

   #132587

   Purpose

   gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

   Depositor

   James Thomson

   Article

   Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

   Insert

   MYH11-gRNA1
   

   Expression

   Bacterial

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-ABE7.8

   Plasmid

   #102917

   Purpose

   expresses ABE7.8 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.8
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HaloTag-WDR5

   Plasmid

   #200880

   Purpose

   Mammalian expression of human WDR5 with N-terminal HaloTag fusion

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   HaloTag-WDR5 (WDR5 Human)
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. KA1042_pPB-hCMV*1-Esrrb-IV

   Plasmid

   #124177

   Purpose

   piggyBac-based Dox-inducible expression of Esrrb

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Esrrb-IRES-Venus (Esrrb Mouse)
   

   Expression

   Mammalian

   Promoter

   tetO-CMVmin

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. non-targeting control gRNA (BRDN0001145694)

   Plasmid

   #80191

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p59sheLL

    Plasmid

    #40628

    Depositor

    Simon Beddows

    Article

    Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

    Inserts

    HPV59 L1
    HPV59 L2
    

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression

    Promoter

    CMV and Polio IRES

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-MCU-D261A

    Plasmid

    #31729

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed D261 to Alanine

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRY2olig-mCh-CLC

    Plasmid

    #60035

    Purpose

    Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-Clathrin Light Chain (CRY2 Mustard Weed, Mouse)
    

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2, A9D*

    Promoter

    CMV

    Available Since

    Oct. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB80-LAMP1-mCherry-iLID

    Plasmid

    #174625

    Purpose

    Optogenetic coupling to lysosomes (LAMP1) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    LAMP1-mCherry-iLID (LAMP1 Human, Synthetic)
    

    Tags

    mCherry-iLID

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del

    Promoter

    Chicken beta-actin

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p420-3

    Plasmid

    #71257

    Purpose

    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

    Insert

    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF minimal

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-MCU-E264A

    Plasmid

    #31730

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed E264 to Alanine

    Promoter

    None

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. p39sheLL

    Plasmid

    #40627

    Depositor

    Simon Beddows

    Article

    Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

    Inserts

    HPV39 L1
    HPV39 L2
    

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression

    Promoter

    CMV and Polio IRES

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223-MCU-E257A

    Plasmid

    #31727

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed E257 to Alanine

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-Syn-TRPV1-P2A-DsRed

    Plasmid

    #200830

    Purpose

    Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-DsRed (Trpv1 Rat)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-neo-TET

    Plasmid

    #51286

    Purpose

    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    U6 (RNU6-1 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    U6 upstream enhancer sequence

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSW002-Pc-TorA(sp)-mTurquoise2

    Plasmid

    #111254

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA(sp)-mTurquoise2
    

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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